Research laboratory for immunity enhancement in humans and domestic animals, Program of Biotechnology, Faculty of Science, Maejo University, Chiang Mai, Thailand.
Front Immunol. 2022 Jul 18;13:929338. doi: 10.3389/fimmu.2022.929338. eCollection 2022.
This study evaluated the antiviral activities and the immunomodulatory effects of Thunb. (HC) ethanolic extracts in response to porcine reproductive and respiratory syndrome virus (PRRSV). In addition, this study evaluated the effects of oral supplementation of HC extract on immune responses to and cross-protective efficacy of PRRSV-1 modified-live virus (MLV) vaccine against the highly pathogenic (HP)-PRRSV-2 challenge. experiments demonstrated that HC extracted in either 50%, 70%, or 95% ethanol (referred to as HC50, HC70, and HC95, respectively) significantly interfered with PRRSV replication in MARC-145 cells. experiments revealed that all HC extracts significantly enhanced mRNA expressions of type I interferon-regulated genes, type I and II interferon (IFN), and pro- and anti-inflammatory cytokines in HP-PRRSV-2-inoculated monocyte-derived macrophages. An experiment included four groups of six pigs (4 weeks old; = 24). Group 1 and group 2 were vaccinated with the PRRSV-1 MLV vaccine at 0 dpv (day post vaccination). Group 2 also received oral administration of HC50 extract at 0-49 dpv. Group 3 received the PRRSV-1 MLV vaccine solvent at 0 dpv, while group 4 served as strict control. Groups 1-3 were challenged intranasally with HP-PRRSV-2 at 28 dpv and immune-related and clinical parameters were monitored weekly until 49 dpv. Compared to group 1, group 2 demonstrated significantly increased IFN regulatory factor 3 mRNA expression of PRRSV-recalled peripheral blood mononuclear cells, and significantly reduced HP-PRRSV-2 viremia. No difference in PRRSV-specific antibody responses, rectal temperature, clinical scores, and average daily weight gain was detected. Our study reports the immunomodulatory and anti-PRRSV potentials of HC extract in PRRSV-1 MLV-vaccinated/HP-PRRSV-2 challenged pigs.
本研究评估了 Thunb.（HC）乙醇提取物对猪繁殖与呼吸综合征病毒（PRRSV）的抗病毒活性和免疫调节作用。此外，本研究还评估了口服补充 HC 提取物对 PRRSV-1 改良活病毒（MLV）疫苗免疫反应和交叉保护效力的影响，以抵抗高致病性（HP）-PRRSV-2 攻毒。实验表明，用 50%、70%或 95%乙醇提取的 HC（分别称为 HC50、HC70 和 HC95）均能显著抑制 MARC-145 细胞中 PRRSV 的复制。实验表明，所有 HC 提取物均能显著增强 HP-PRRSV-2 感染的单核细胞衍生巨噬细胞中 I 型干扰素调节基因、I 型和 II 型干扰素（IFN）以及促炎和抗炎细胞因子的 mRNA 表达。一项实验包括 4 组 6 头猪（4 周龄；n = 24）。第 1 组和第 2 组于 0 dpv（接种后第 0 天）接种 PRRSV-1 MLV 疫苗。第 2 组还在 0-49 dpv 时口服给予 HC50 提取物。第 3 组于 0 dpv 时接受 PRRSV-1 MLV 疫苗溶剂，第 4 组作为严格对照。第 1-3 组于 28 dpv 经鼻腔感染 HP-PRRSV-2，直至 49 dpv 每周监测免疫相关和临床参数。与第 1 组相比，第 2 组 PRRSV 回忆性外周血单核细胞中 IFN 调节因子 3 mRNA 表达显著增加，HP-PRRSV-2 病毒血症显著降低。PRRSV 特异性抗体反应、直肠温度、临床评分和平均日增重无差异。本研究报道了 HC 提取物在 PRRSV-1 MLV 疫苗接种/HP-PRRSV-2 攻毒猪中的免疫调节和抗 PRRSV 潜力。
