JMC Scientific Consulting Ltd, Egham, UK.
DSM Nutritional Products Ltd, R&D Personal Care, Kaiseraugst, Switzerland.
Int J Cosmet Sci. 2022 Dec;44(6):663-671. doi: 10.1111/ics.12809. Epub 2022 Sep 21.
Microscopy is widely used during the development and testing of topical formulations; however, it often lacks the ability to be chemically specific with regard to what is being imaged. This article describes how moving outside of the visible light region and into different parts of the ultraviolet (UV) spectrum enables differently UV absorbing components in topical emulsions to be directly visualized using optical transmission microscopy.
Optical transmission microscopy of different sunscreen emulsions was carried out using a custom-built microscope, imaging in the UVB (313 nm), UVA (365 nm) and visible light (546 nm) and with different magnifications.
By using light of different wavelengths, direct visualization of different UV absorbing ingredients within the product emulsion using optical transmission microscopy has been performed and the locations of the UV absorbing actives in the formulations imaged.
Microscopy has long been a valuable tool for the skin researcher, providing structural information about the products and how they perform. By moving outside of the spectral region of visible light and into the UV, it has been possible for the first time to directly image different SPF ingredients within topical formulations using optical microscopy.
在开发和测试局部制剂时，显微镜被广泛应用；然而，它通常缺乏对成像内容进行化学特异性分析的能力。本文介绍了如何将显微镜的应用范围从可见光区域扩展到紫外线（UV）光谱的不同部分，以便使用光学透射显微镜直接观察局部乳液中不同的 UV 吸收成分。
使用定制的显微镜对不同的防晒霜乳液进行光学透射显微镜检查，在 UVB（313nm）、UVA（365nm）和可见光（546nm）下以及不同的放大倍数下进行成像。
通过使用不同波长的光，已经可以使用光学透射显微镜直接观察产品乳液中不同的 UV 吸收成分，并对制剂中 UV 吸收剂的位置进行成像。
显微镜一直是皮肤研究人员的宝贵工具，它提供了有关产品及其性能的结构信息。通过将光谱范围从可见光扩展到紫外线，现在第一次可以使用光学显微镜直接观察局部制剂中不同的 SPF 成分。
