Thuricins: Novel Leaderless Bacteriocins with Potent Antimicrobial Activity Against Gram-Positive Foodborne Pathogens.

Bacteriocins are bacterial-derived peptides that exhibit antimicrobial activity and can be used as food preservatives. Here, using the indicator strain CMCC63301, we screened and identified a LX43 strain that exhibits potent antimicrobial activity and harbors a putative leaderless bacteriocin gene cluster ( gene cluster). Five novel leaderless bacteriocins, thuricin A1, A2, A3, A4, and A5, encoded by the gene cluster, were purified and identified. Thuricin A5 was regarded as a representative and showed remarkable antimicrobial activity against foodborne pathogens , , , and , likely by damaging their cell envelope. Moreover, thuricin A5 displayed good thermal and pH stability, with no hemolytic activity and cytotoxicity, indicating its wide applicability and biosafety. Furthermore, thuricin A5 effectively inhibited or eradicated foodborne pathogens in skim milk at 25 °C in a dose-dependent manner, affirming its potential for use as a novel biopreservative in foods.