Cherif A, Ouzari H, Daffonchio D, Cherif H, Ben Slama K, Hassen A, Jaoua S, Boudabous A
Laboratoire de Microbiologie, Département de Biologie, Faculté des Sciences de Tunis, Tunisia.
Lett Appl Microbiol. 2001 Apr;32(4):243-7. doi: 10.1046/j.1472-765x.2001.00898.x.
Detection and identification of new antagonistic activities towards Bacillus cereus and relatives.
Twenty Bacillus thuringiensis strains were screened for their capacity to express bacteriocin-like agents. Strain BMG1.7, isolated from soil, showed an antagonistic activity called thuricin 7. Thuricin 7 was active against several species of the genus Bacillus, including three of the four known B. thuringiensis/B. cereus bacteriocin producers, as well as against Streptococcus pyogenes and Listeria monocytogenes strains. Antimicrobial activity was lost after treatment with proteinase K. The active protein had an apparent molecular weight of 11.6 kDa, and was secreted at the end of the exponential growth phase. Thuricin 7 retained 55% of the activity after incubation at 98 degrees C for 30 min. The mode of action of thuricin 7 was shown to be bactericidal and bacteriolytic.
Thuricin 7 is a novel bacteriocin produced by a newly isolated Bacillus thuringiensis strain BMG1.7.
The characteristics of thuricin 7 indicate that it is a new bacteriocin which may have interesting biotechnological applications due to its relatively large activity spectrum.
检测和鉴定对蜡样芽孢杆菌及其相关菌的新拮抗活性。
筛选了20株苏云金芽孢杆菌菌株表达类细菌素的能力。从土壤中分离得到的菌株BMG1.7表现出一种名为thuricin 7的拮抗活性。thuricin 7对几种芽孢杆菌属的细菌具有活性，包括已知的4种苏云金芽孢杆菌/蜡样芽孢杆菌细菌素产生菌中的3种，以及化脓性链球菌和单核细胞增生李斯特菌菌株。用蛋白酶K处理后抗菌活性丧失。活性蛋白的表观分子量为11.6 kDa，在指数生长期结束时分泌。thuricin 7在98℃孵育30分钟后仍保留55%的活性。thuricin 7的作用方式显示为杀菌和溶菌。
thuricin 7是由新分离的苏云金芽孢杆菌菌株BMG1.7产生的一种新型细菌素。
thuricin 7的特性表明它是一种新型细菌素，由于其相对较宽的活性谱，可能具有有趣的生物技术应用。
