School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, No. 10 Poyang Lake Road, Jinghai District, Tianjin, 301617, China.
Tianjin traditional Chinese Medicine Decoction Piece Factory Co., Ltd., Tianjin, 300000, China.
Chem Biodivers. 2022 Sep;19(9):e202200362. doi: 10.1002/cbdv.202200362. Epub 2022 Aug 19.
The quality of the root bark of Morus alba L. (SBP) herbs currently circulating in the market is variable. In order to ensure clinical effectiveness, a high-performance liquid chromatography (HPLC) fingerprinting method combined with chemical pattern recognition should be established to control the quality of SBP herbs. The differences of 23 batches of SBP were analyzed by exploratory cluster analysis based on shared fingerprint peak data, and the results indicated that the processing method to remove the cork layer from SBP materials is an important influencing factor on SBP quality. Principal component analysis indicated that SBP samples with the cork layer removed can be clearly distinguished from samples without cork layer removal. The potential chemical markers (kuwanon G, morusin and oxyresveratrol) were screened by partial least squares discriminant analysis. Finally, the contents of the main components were determined, indicating that the processing method of SBP materials can affect content of bioactive ingredients and that cork layer removal leads to a more uniform chemometric profile. The HPLC-based chemometrics approach described here will support the development of quality standards in SBP products.
市售桑白皮药材的质量参差不齐。为保证临床疗效，应建立高效液相色谱（HPLC）指纹图谱结合化学模式识别的方法来控制桑白皮药材的质量。基于共有指纹峰数据，采用探索性聚类分析对 23 批桑白皮进行了差异分析，结果表明，桑白皮材料去栓皮的加工方法是影响桑白皮质量的重要因素。主成分分析表明，去栓皮的桑白皮样品与未去栓皮的样品可以明显区分。采用偏最小二乘判别分析筛选潜在的化学标志物（桑酮 G、桑素和氧化白藜芦醇）。最后，测定了主要成分的含量，表明桑白皮材料的加工方法会影响生物活性成分的含量，而去栓皮会导致更均匀的化学计量特征。本文所描述的基于 HPLC 的化学计量学方法将支持桑白皮产品质量标准的制定。
