Department of Biological Sciences and Biotechnology, College of Natural Science, Chungbuk National Universitygrid.254229.a, Cheongju, Republic of Korea.
Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National Universitygrid.254229.a, Cheongju, Republic of Korea.
Microbiol Spectr. 2022 Aug 31;10(4):e0125622. doi: 10.1128/spectrum.01256-22. Epub 2022 Aug 4.
Shaan virus (ShaV), a novel species of the genus , family , was isolated from an insectivore bat (Miniopterus schreibersii) in Korea in 2016. ShaV particles contain a hemagglutinin-neuraminidase (HN) glycoprotein in their envelope that allows the virus to target cells. Typically, diverse paramyxoviruses with HN glycoprotein are reported to interact predominantly with sialic acids, but there are no studies of receptors for ShaV. In this study, the identification of potential receptors for ShaV was demonstrated using sialidase treatments, glycan microarray, magnetic bead-based virus binding assay, and neuraminidase inhibitor treatments. Pretreatment of MARC-145 cells with sialidase, which cleaves α2,3-linked sialic acids, showed higher inhibition of viral infection than α2,6-linked-specific sialidase. These data were supported by the binding of ShaV to predominantly α2,3-linked sialylated glycans in the screening of sialyl linkage patterns through glycan microarray. To further confirm the direct interaction between ShaV and α2,3-linked sialic acids, ShaV was incubated with α2,3- or α2,6-linked sialylated glycans conjugated to magnetic beads, and binding signals were detected only for α2,3-linked sialylated glycans. In addition, the potential of sialic acids as a receptor was demonstrated by the viral replication inhibitory effect of the neuraminidase inhibitor 2,3-dehydro-2-deoxy--acetylneuraminicacid (DANA) in the mature virion release steps. Collectively, these results support that α2,3-linked sialic acids are the potential receptor for ShaV infection in MARC-145 cells. Bats host major mammalian paramyxoviruses, and novel paramyxoviruses are increasingly being reported around the world. Shaan virus (ShaV), from the genus , family , has a potential attachment glycoprotein, HN. Here, we identify that ShaV binds to sialic acid and demonstrate that α2,3-linked sialic acids are the potential receptor for ShaV infection. The presented data regarding ShaV receptor specificity will enable studies into the viral tropism for the host and contribute to the development of new antiviral strategies targeting viral receptors.
沙阿病毒（ShaV）是一种新型的属，科病毒，于 2016 年在韩国的一种食虫蝙蝠（Miniopterus schreibersii）中分离得到。ShaV 颗粒的包膜中含有血凝素-神经氨酸酶（HN）糖蛋白，使病毒能够靶向细胞。通常，具有 HN 糖蛋白的多种副粘病毒主要与唾液酸相互作用，但尚未研究 ShaV 的受体。在这项研究中，使用唾液酸酶处理、聚糖微阵列、基于磁珠的病毒结合测定和神经氨酸酶抑制剂处理来证明 ShaV 的潜在受体的鉴定。用唾液酸酶（可裂解α2,3 连接的唾液酸）预处理 MARC-145 细胞显示出比α2,6 特异性唾液酸酶更高的抑制病毒感染的作用。这些数据通过聚糖微阵列筛选唾液酸连接模式得到支持，表明 ShaV 与主要为α2,3 连接的唾液酸化糖结合。为了进一步确认 ShaV 与α2,3 连接的唾液酸之间的直接相互作用，将 ShaV 与连接到磁珠上的α2,3 或α2,6 连接的唾液酸化糖孵育，并仅检测到α2,3 连接的唾液酸化糖的结合信号。此外，神经氨酸酶抑制剂 2,3-去氢-2-脱氧--乙酰神经氨酸（DANA）在成熟病毒释放步骤中对病毒复制的抑制作用证明了唾液酸作为受体的潜力。总的来说，这些结果支持α2,3 连接的唾液酸是 MARC-145 细胞中 ShaV 感染的潜在受体。蝙蝠宿主主要的哺乳动物副粘病毒，并且世界各地越来越多地报道新型副粘病毒。沙阿病毒（ShaV），属于科，具有潜在的附着糖蛋白 HN。在这里，我们确定 ShaV 与唾液酸结合，并证明α2,3 连接的唾液酸是 ShaV 感染的潜在受体。关于 ShaV 受体特异性的研究数据将使宿主的病毒嗜性研究成为可能，并有助于开发针对病毒受体的新抗病毒策略。
