Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic.
Department of Diseases of Zoo Animals, Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic.
Arch Virol. 2022 Nov;167(11):2213-2222. doi: 10.1007/s00705-022-05548-x. Epub 2022 Aug 4.
Lake Sinai virus (LSV) is one of over 20 honey bee viruses. Variants of LSV have been classified as members of two officially recognised species, Lake Sinai virus 1 and Lake Sinai virus 2. However, there are currently a limited number of whole-genome sequences, and the genetic variability of the virus indicates that additional species may need to be established. Extracted nucleic acid of 209 honey bee samples was screened by PCR for 11 honey bee viruses. LSV was the third most abundant virus (36.9% of positive samples), after Apis mellifera filamentous virus (72.2%) and deformed wing virus (52.5%). LSV-positive samples were analyzed further by PCR with primers targeting the region encoding the viral RNA-dependent RNA polymerase. Subsequently, the PCR products were sequenced, and the resulting sequences were used for a first round of phylogenetic analysis. Based on those results, several isolates were selected for whole-genome sequencing, and the complete genome sequences were used for additional phylogenetic analysis. The results indicated the presence of at least three genetically distinct groups of LSV in the Czech Republic, the most prevalent one being related to LSV 2 but too dissimilar to be considered a member of the same species. Two sequences of a major LSV strain cluster native to the Czech Republic were determined, representing the first Czech LSV strains published to date.
西奈湖病毒（LSV）是超过 20 种蜜蜂病毒之一。LSV 的变体已被分类为两种正式认可的物种，即西奈湖病毒 1 和西奈湖病毒 2。然而，目前全基因组序列的数量有限，病毒的遗传变异性表明可能需要建立更多的物种。通过 PCR 对 209 个蜜蜂样本的提取核酸进行了 11 种蜜蜂病毒的筛查。LSV 是第三丰富的病毒（36.9%的阳性样本），仅次于绵蚜丝状病毒（72.2%）和变形翅膀病毒（52.5%）。对 LSV 阳性样本进一步用针对编码病毒 RNA 依赖性 RNA 聚合酶的区域的引物进行 PCR。随后，对 PCR 产物进行测序，并将获得的序列用于第一轮系统发育分析。根据这些结果，选择了几个分离株进行全基因组测序，并将完整的基因组序列用于进一步的系统发育分析。结果表明，在捷克共和国至少存在三个遗传上不同的 LSV 群体，最流行的一个与 LSV 2 有关，但与 LSV 2 差异太大，不能被认为是同一物种的成员。确定了两个源自捷克共和国的主要 LSV 株的序列，这是迄今为止首次发表的捷克 LSV 株。
