Phokasem Patcharin, Sinpoo Chainarong, Attasopa Korrawat, Krongdang Sasiprapa, Chantaphanwattana Thunyarat, Ling Tial C, Pettis Jeffery S, Chantawannakul Panuwan, Chaimanee Veeranan, Disayathanoowat Terd
Bee Protection Laboratory, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Environmental Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Life (Basel). 2023 Feb 3;13(2):438. doi: 10.3390/life13020438.
Widespread parasites, along with emerging threats, globalization, and climate change, have greatly affected honey bees' health, leading to colony losses worldwide. In this study, we investigated the detection of biotic stressors (i.e., viruses, microsporidian, bacteria, and fungi) in by surveying the colonies across different regions of Thailand (Chiang Mai in the north, Nong Khai and Khon Kaen in the northeast, and Chumphon and Surat Thani in the south, in addition to the Samui and Pha-ngan islands). In this study, we detected ABPV, BQCV, LSV, and in samples through RT-PCR. ABPV was only detected from the samples of Chiang Mai, whereas we found BQCV only in those from Chumphon. LSV was detected only in the samples from the Samui and Pha-ngan islands, where historically no managed bees are known. was found in all of the regions except for Nong Khai and Khon Kaen in northeastern Thailand. and were not detected in any of the samples in this survey. The phylogenetic tree analysis of the pathogens provided insights into the pathogens' movements and their distribution ranges across different landscapes, indicating the flow of pathogens among the honey bees. Here, we describe the presence of emerging pathogens in the Asian honey bee as a valuable step in our understanding of these pathogens in terms of the decline in eastern honey bee populations.
广泛存在的寄生虫，连同新出现的威胁、全球化和气候变化，极大地影响了蜜蜂的健康，导致全球范围内蜂群损失。在本研究中，我们通过调查泰国不同地区（北部的清迈、东北部的廊开和孔敬，南部的春蓬和素叻他尼，以及苏梅岛和帕岸岛）的蜂群，来研究生物应激源（即病毒、微孢子虫、细菌和真菌）的检测情况。在本研究中，我们通过逆转录聚合酶链反应（RT-PCR）在样本中检测到了急性蜜蜂麻痹病毒（ABPV）、黑蜂王台病毒（BQCV）、慢蜜蜂麻痹病毒（LSV）和[此处原文缺失一种病原体名称]。ABPV仅在清迈的样本中被检测到，而BQCV仅在春蓬的样本中被发现。LSV仅在苏梅岛和帕岸岛的样本中被检测到，在历史上那里没有已知的人工养殖蜜蜂。[此处原文缺失一种病原体名称]在泰国东北部廊开和孔敬以外的所有地区都被发现。在本次调查的所有样本中均未检测到[此处原文缺失两种病原体名称]。对病原体的系统发育树分析为病原体的传播及其在不同区域的分布范围提供了见解，表明病原体在蜜蜂之间的传播情况。在此，我们描述了亚洲蜜蜂中出现的新病原体，这是我们在了解这些病原体对东方蜜蜂种群数量下降影响方面迈出的重要一步。
