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Determination of 4-hydroxy-3-methoxymandelic acid in urine using HPLC with electrochemical detection.

作者信息

Palfrey S M

出版信息

Ann Clin Biochem. 1987 Mar;24 ( Pt 2):172-6. doi: 10.1177/000456328702400208.

DOI:10.1177/000456328702400208
PMID:3592567
Abstract

A method is described for the assay of 4-hydroxy-3-methoxymandelic acid (HMMA) in urine using HPLC coupled with electrochemical detection. In this method acidified urine is extracted with ethyl acetate which is then washed with the chromatography solvent to back extract HMMA. The solvent is a pH 5.0 citrate buffer. At this pH, HMMA separates clearly from all other electroactive compounds present in urine.

摘要

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