Moleman P, Borstrok J J
Clin Chem. 1983 May;29(5):878-81.
We describe an improved method for the assay of urinary vanillylmandelic acid by "high-performance" liquid chromatography, with electrochemical detection. A 1-mL aliquot of urine is acidified and extracted with ethyl acetate, then the ethyl acetate extract is extracted with phosphate buffer, pH 8.5. An acidified aliquot of the phosphate extract is injected into a reversed-phase column and vanillylmandelic acid is detected electrochemically. The between-day CV is 5-6% for concentrations ranging from 10 to 75 mumol/L. We also discuss the critical steps for extraction and calibration.
我们描述了一种通过“高效”液相色谱法并结合电化学检测来测定尿香草扁桃酸的改进方法。取1 mL尿液等分试样酸化后用乙酸乙酯萃取，然后乙酸乙酯萃取液再用pH 8.5的磷酸盐缓冲液萃取。取磷酸盐萃取液的酸化等分试样注入反相柱，通过电化学检测香草扁桃酸。浓度范围为10至75 μmol/L时，日间变异系数为5 - 6%。我们还讨论了萃取和校准的关键步骤。
