Biological variation of cardiovascular biochemical markers in patients with Type 2 Diabetes Mellitus.

BACKGROUND

Type 2 diabetes mellitus (T2DM) is a well-established risk factor for cardiovascular diseases. We aimed to identify the biological variation of ten cardiovascular biochemical markers in T2DM patients to aid in their interpretation.

METHODS

Blood samples for evaluating ten biomarkers were collected biweekly from 23 T2DM patients (10 men, 13 women) for three months. The analytical variability and variations of within-subject (CV) and between-subject (CV) levels were calculated, as well as the analytical performance specifications, reference change value (RCV), and index of individuality (II).

RESULTS

The levels of total cholesterol (CHOL), apolipoprotein A (apoA), homocysteine (HCY), high-sensitivity troponin T (hsTnT) and N-terminal pro-brain natriuretic peptide (NT-proBNP) differed between males and females (P < 0.05). The CVs or CVs of the biomakers were higher than those of healthy participants in Westgard online database, except for hsTnT. Triglyceride (TG), lipoprotein (a) [Lp(a)] and NT-proBNP had relatively high CV, CV and RCV, whereas CHOL, high-density lipoprotein cholesterol (HDL-C), apoA and HCY showed low variation. Moreover, the II of HDL-C, LP(a), apoA, HCY and hsTnT was <0.6 and other biochemical markers was between 0.6 and 1.4.

CONCLUSION

The cardiovascular biochemical markers in T2DM patients showed higher CV or CV, except for hsTnT. ApoA had the lowest CV and CV values. Population-based reference intervals should be used with caution in clinical decision-making for T2DM patients.