泉生根瘤菌分泌的一种水溶性且具有抗菌活性的β-葡聚糖的结构特征。
Structural characterization of a water-soluble and antimicrobial β-glucan secreted by Rhizobium pusense.
机构信息
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
出版信息
J Biosci Bioeng. 2022 Oct;134(4):318-325. doi: 10.1016/j.jbiosc.2022.06.015. Epub 2022 Aug 1.
In this study, a bacterium with the ability to extracellularly produce a water-soluble polysaccharide (with high molecular mass of 743 kDa) was obtained from saline soils. This strain named as ZB01 was identified as Rhizobium punsense by 16S rRNA sequence analysis. The monomer composition and structure of extracellular polysaccharides were investigated by high-performance anion-exchange chromatography-pulsed amperometric detector, Fourier-transform infrared, methylation and nuclear magnetic resonance spectroscopy analysis. In addition, the morphological characterization of extracellular polysaccharides was performed by scanning electron microscopy analysis. As a result, the polysaccharide is found to be a novel water-soluble type of glucan most likely consisting of repeating two β-d-Glcp-(1 → 3) and one α-d-Glcp-(1 → 3). Furthermore, it showed pronounced antimicrobial effects against Escherichia coli and Bacillus subtilis, suggesting it has the potential to be used as a natural antibacterial agent in the future.
在这项研究中，从盐土中获得了一种能够胞外产生水溶性多糖（分子量高达 743 kDa）的细菌。该菌株被命名为 ZB01，通过 16S rRNA 序列分析鉴定为根瘤菌 punsense。通过高效阴离子交换色谱-脉冲安培检测器、傅里叶变换红外光谱、甲基化和核磁共振波谱分析研究了胞外多糖的单体组成和结构。此外，通过扫描电子显微镜分析对胞外多糖的形态特征进行了研究。结果表明，该多糖是一种新型的水溶性葡聚糖，很可能由重复的两个β-d-Glcp-(1 → 3)和一个α-d-Glcp-(1 → 3)组成。此外，它对大肠杆菌和枯草芽孢杆菌表现出明显的抗菌作用，表明它有可能在未来被用作天然抗菌剂。
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