Department of Chemistry & Chemical Technology, Vidyasagar University, Midnapore 721 102, West Bengal, India.
Carbohydr Res. 2010 Dec 10;345(18):2657-63. doi: 10.1016/j.carres.2010.10.005. Epub 2010 Oct 12.
Two different glucans (PS-I, water-soluble; and PS-II, water-insoluble) were isolated from the alkaline extract of fruit bodies of an edible mushroom Calocybe indica. On the basis of acid hydrolysis, methylation analysis, periodate oxidation, and NMR analysis ((1)H, (13)C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit of these polysaccharides were established as: PS-I: →6)-β-D-Glcp-(1→6)-β-D-glcp-(1→6)-)-β-D-Glcp-(1→ α-D=Glcp (Water-soluble glucan). PS-II: →3)-β-D-Glcp-(1→3)-β-D-glcp-(1→3)-)-β-D-Glcp-(1→3)-β-D-Glcp-(1→ β-D-Glcp (Water-insoluble glucan, Calocyban).
两种不同的葡聚糖（PS-I，水溶性；PS-II，水不溶性）从食用蘑菇鸡油菌的碱性提取物中分离得到。根据酸水解、甲基化分析、高碘酸盐氧化和 NMR 分析（（1）H、（13）C、DEPT-135、TOCSY、DQF-COSY、NOESY、ROESY、HMQC 和 HMBC），这些多糖重复单元的结构被确定为：PS-I：→6））-β-D-Glcp-（1→6））-β-D-glcp-（1→6））-β-D-Glcp-（1→α-D=Glcp（水溶性葡聚糖）。PS-II：→3））-β-D-Glcp-（1→3））-β-D-glcp-（1→3））-β-D-Glcp-（1→3）-β-D-Glcp-（1→β-D-Glcp（水不溶性葡聚糖，Calocyban）。
