U.S. Army Engineer Research and Development Center, Environmental Laboratory, Vicksburg, MS, USA.
Institute for Genomics, Biocomputing & Biotechnology (IGBB), Mississippi State University, Starkville, MS, USA.
Sci Data. 2022 Aug 4;9(1):476. doi: 10.1038/s41597-022-01553-6.
Contaminants of Emerging Concern (CECs) can be measured in waters across the United States, including the tributaries of the Great Lakes. The extent to which these contaminants affect gene expression in aquatic wildlife is unclear. This dataset presents the full hepatic transcriptomes of laboratory-reared fathead minnows (Pimephales promelas) caged at multiple sites within the Milwaukee Estuary Area of Concern and control sites. Following 4 days of in situ exposure, liver tissue was removed from males at each site for RNA extraction and sequencing, yielding a total of 116 samples from which libraries were prepared, pooled, and sequenced. For each exposure site, 179 chemical analytes were also assessed. These data were created with the intention of inviting research on possible transcriptomic changes observed in aquatic species exposed to CECs. Access to both full sequencing reads of animal samples as well as water contaminant data across multiple Great Lakes sites will allow others to explore the health of these ecosystems in support of the aims of the Great Lakes Restoration Initiative.
美国各地的水域都可以检测到新兴关注污染物（CECs），包括大湖的支流。这些污染物对水生野生动物基因表达的影响程度尚不清楚。本数据集提供了在密尔沃基关注区和对照点的多个地点饲养的实验室胖头小鱼（Pimephales promelas）的完整肝脏转录组。在现场暴露 4 天后，从每个地点的雄性鱼体中取出肝脏组织进行 RNA 提取和测序，共获得 116 个样本，从中制备、混合和测序文库。对于每个暴露地点，还评估了 179 种化学分析物。创建这些数据的目的是邀请研究人员研究暴露于 CEC 的水生物种中观察到的可能的转录组变化。可以访问动物样本的完整测序reads 以及多个大湖站点的水污染物数据，这将使其他人能够探索这些生态系统的健康状况，以支持大湖恢复倡议的目标。
