Thrash Adam, Arick Mark, Peterson Daniel G
Institute for Genomics, Biocomputing & Biotechnology, Mississippi State University, Mississippi State, MS 39762, USA.
Institute for Genomics, Biocomputing & Biotechnology, Mississippi State University, Mississippi State, MS 39762, USA.
Anal Biochem. 2018 May 1;548:38-43. doi: 10.1016/j.ab.2018.01.028. Epub 2018 Feb 1.
The quality of data generated by high-throughput DNA sequencing tools must be rapidly assessed in order to determine how useful the data may be in making biological discoveries; higher quality data leads to more confident results and conclusions. Due to the ever-increasing size of data sets and the importance of rapid quality assessment, tools that analyze sequencing data should quickly produce easily interpretable graphics. Quack addresses these issues by generating information-dense visualizations from FASTQ files at a speed far surpassing other publicly available quality assurance tools in a manner independent of sequencing technology.
必须迅速评估高通量DNA测序工具生成的数据质量，以确定这些数据在进行生物学发现时可能有多大用处；更高质量的数据会带来更可靠的结果和结论。由于数据集规模不断增大以及快速质量评估的重要性，分析测序数据的工具应能快速生成易于解读的图形。Quack通过以远超其他公开可用质量保证工具的速度，从FASTQ文件生成信息密集型可视化结果来解决这些问题，且其方式独立于测序技术。
