Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Miaoli, Taiwan.
Department of Biomedical Imaging and Radiological Sciences, Taipei Branch, National Yang Ming Chiao Tung University, Taipei, Taiwan.
BMC Med Imaging. 2022 Aug 4;22(1):136. doi: 10.1186/s12880-022-00867-6.
Altered neural activity based on the fractional amplitude of low-frequency fluctuations (fALFF) has been reported in patients with diabetes. However, whether fALFF can differentiate healthy controls from diabetic animals under anesthesia remains unclear. The study aimed to elucidate the changes in fALFF in a rat model of diabetes under isoflurane anesthesia.
The first group of rats (n = 5) received a single intraperitoneal injection of 70 mg/kg streptozotocin (STZ) to cause the development of diabetes. The second group of rats (n = 7) received a single intraperitoneal injection of the same volume of solvent. Resting-state functional magnetic resonance imaging was used to assess brain activity at 4 weeks after STZ or solvent administration.
Compared to the healthy control animals, rats with diabetes showed significantly decreased fALFF in various brain regions, including the cingulate cortex, somatosensory cortex, insula, and striatum (all P < 0.05). The decreased fALFF suggests the aberrant neural activities in the diabetic rats. No regions were detected in which the control group had a lower fALFF than that in the diabetes group.
The results of this study demonstrated that the fALFF could be used to differentiate healthy controls from diabetic animals, providing meaningful information regarding the neurological pathophysiology of diabetes in animal models.
已有研究报道糖尿病患者的低频振幅分数（fALFF）存在神经活动改变。然而，在麻醉状态下，fALFF 是否可用于区分健康对照和糖尿病动物尚不清楚。本研究旨在阐明异氟醚麻醉下糖尿病大鼠模型中 fALFF 的变化。
第一组大鼠（n=5）单次腹腔注射 70mg/kg 链脲佐菌素（STZ）以诱发糖尿病。第二组大鼠（n=7）单次腹腔注射相同体积的溶剂。STZ 或溶剂给药 4 周后，采用静息态功能磁共振成像评估大脑活动。
与健康对照组相比，糖尿病大鼠的大脑多个区域的 fALFF 明显降低，包括扣带回皮质、躯体感觉皮质、岛叶和纹状体（均 P<0.05）。fALFF 的降低表明糖尿病大鼠的神经活动异常。未检测到对照组 fALFF 低于糖尿病组的区域。
本研究结果表明，fALFF 可用于区分健康对照和糖尿病动物，为动物模型中糖尿病的神经病理生理学提供了有意义的信息。
