Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi, Vietnam.
Department of Chemical Engineering, Hanoi University of Industry, 298 Cau Dien, North Tu Liem, Hanoi, Vietnam.
Chem Biodivers. 2022 Sep;19(9):e202200376. doi: 10.1002/cbdv.202200376. Epub 2022 Aug 19.
Six new polyoxygenated xanthones, garcicowanones F-H (1-3), norcowanol A-B (4-5), and garcinone F (6) along with twelve known compounds 7-18 were obtained from the latex of Garcinia cowa Roxb. ex Choisy. All new compounds have a 1,3,7-trioxygenated or 1,3,6,7-tetraoxygenated xanthone nucleus and differ from majority of xanthones from G. cowa by hydrated side chains. Compounds 1, 7, 8 and 18 exhibited significant neuroprotective effects on glutamate-mediated hippocampal neuronal HT22 cell death. In particular, compound 1 exhibited the most potent neuroprotective effect with >80 % cell viability in the concentration range of 2.9-115 μM. Further studies on compound 1 showed that it decreased cellular Ca influx and inhibits cellular reactive oxygen species generation in HT22 cells. A Western blot analysis showed that MAPK phosphorylation, Bax, and AIF translocation dramatically increased upon treatment with 5 mM glutamate and decreased upon a co-treatment with compound 1.
从 Garcinia cowa Roxb. ex Choisy 的乳汁中分离得到了 6 个新的多氧化黄烷酮，即 garcicowanones F-H（1-3）、norcowanol A-B（4-5）和 garcinone F（6），以及 12 个已知化合物 7-18。所有新化合物都具有 1,3,7-三氧化或 1,3,6,7-四氧化黄烷酮核，与大多数来自 G. cowa 的黄烷酮不同之处在于其侧链水合。化合物 1、7、8 和 18 对谷氨酸介导的海马神经元 HT22 细胞死亡表现出显著的神经保护作用。特别是化合物 1 在 2.9-115 μM 的浓度范围内表现出最强的神经保护作用，细胞活力超过 80%。对化合物 1 的进一步研究表明，它可降低 HT22 细胞中的细胞内 Ca2+内流并抑制细胞内活性氧的生成。Western blot 分析表明，用 5 mM 谷氨酸处理后，MAPK 磷酸化、Bax 和 AIF 易位显著增加，而与化合物 1 共同处理后则降低。
