Byarugaba W
Chromosoma. 1978 Jul 31;67(3):275-83. doi: 10.1007/BF02569040.
The enzymes pepsin, alpha-chymotrypsin, trypsin, RNase and DNase were applied to preparations of human metaphase chromosomes before staining to study whether dissociable materials related to the formation of G-, Q- and C-bands would be seen. Treatment with active pepsin but not the other enzymes revealed material with ribonucleo-protein properties which dissociated from the chromosomes and formed a halo.--Lateral extensions from the chromatids stretched to the rim of the halo and appeared at positions corresponding to G-bands. A G-band may be defined as a ring of stable chromatid-matrix binding at positions where the chromatids coil to form lateral extensions.
在染色前，将胃蛋白酶、α-胰凝乳蛋白酶、胰蛋白酶、核糖核酸酶和脱氧核糖核酸酶应用于人类中期染色体标本，以研究是否能观察到与G带、Q带和C带形成相关的可解离物质。用活性胃蛋白酶处理而非其他酶处理后，发现有具有核糖核蛋白特性的物质从染色体上解离并形成晕圈。染色单体的横向延伸伸展到晕圈边缘，并出现在与G带相对应的位置。G带可定义为染色单体在盘绕形成横向延伸的位置处与基质稳定结合的环。
