Xu X, Wu M
Chromosoma. 1983;88(3):237-40. doi: 10.1007/BF00285626.
Trypsin-treated human metaphase chromosomes stained with Giemsa and uranyl acetate showed clear, reproducible band structures under the transmission electron microscope (TEM). The banding pattern observed with TEM corresponded very closely to the G-band pattern visualized by light microscopy. The TEM images were used for karyotype analyses. Trypsin-treated chromosomes stained with uranyl acetate alone also showed clear G-bands under TEM. Shadow casting in addition to uranyl acetate staining revealed more structural detail of the chromosomes. Chromosome fibers, 200 A-300 A in diameter, were observed in the interband regions. Most chromosomes showed the major G-bands under the higher TEM magnification without any trypsin treatment.
用胰蛋白酶处理过的人类中期染色体，经吉姆萨染色和醋酸铀染色后，在透射电子显微镜（TEM）下呈现出清晰、可重复的带型结构。TEM观察到的带型模式与光学显微镜下观察到的G带模式非常吻合。TEM图像用于核型分析。仅用醋酸铀染色的胰蛋白酶处理过的染色体在TEM下也显示出清晰的G带。除醋酸铀染色外，投影显示出染色体更多的结构细节。在带间区域观察到直径为200埃至300埃的染色体纤维。大多数染色体在较高的TEM放大倍数下，未经任何胰蛋白酶处理就显示出主要的G带。
