锌指转录因子与其同源启动子结合的蛋白-DNA 结合分析方案。
Protocols for protein-DNA binding analysis of a zinc finger transcription factor bound to its cognate promoter.
机构信息
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230026, China; Ministry of Education Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science and Technology of China, Hefei, China.
出版信息
STAR Protoc. 2022 Jul 31;3(3):101598. doi: 10.1016/j.xpro.2022.101598. eCollection 2022 Sep 16.
Here, we describe protocols to interrogate the binding of the zinc fingers of the transcription factor ZBTB7A to the fetal () promoter. We detail the steps for performing electrophoretic mobility shift assays (EMSAs), X-ray crystallography, and isothermal titration calorimetry (ITC) to explore this interaction. These techniques could readily be applied to the structural studies of other zinc finger transcription factors and cognate DNA sequences. For complete details on the use and execution of this protocol, please refer to Yang et al. (2021).
在这里，我们描述了探究转录因子 ZBTB7A 的锌指与胎儿 () 启动子结合的实验方案。我们详细介绍了进行电泳迁移率变动分析 (EMSA)、X 射线晶体学和等温热力学滴定 (ITC) 以探索这种相互作用的步骤。这些技术可以很容易地应用于其他锌指转录因子和同源 DNA 序列的结构研究。有关此方案使用和执行的完整详细信息，请参阅 Yang 等人。(2021)。
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