Yang Hyun, Lee Sang R, Jo Seong Lae, Kim Ae-Hyang, Kim Eun-Ryoung, Qu Fan, Hong Eui-Ju, Lee Hye Won
KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon, South Korea.
College of Veterinary Medicine, Chungnam National University, Daejeon, South Korea.
Front Pharmacol. 2022 Jul 19;13:905191. doi: 10.3389/fphar.2022.905191. eCollection 2022.
Polycystic Ovarian Syndrome (PCOS) is known to be an endocrine state that is characterized by oligomenorrhea, hyperandrogenism, and highly cystic follicles in the ovaries. The use of food ingredients and traditional medicine in Asian countries is well known, and previous studies have shown that K. [Alariaceae] (EC) is able to alleviate PCOS symptoms. D-Chiro-inositol (DCI) administration in pathologies where steroid biosynthesis is a crucial factor, i.e., PCOS, has provided satisfactory results. Therefore, we studied the synergistic effects of the two previously known active compounds. In rats with letrozole-induced PCOS, we focused on alternative therapies using EC and/or DCI extracts to alleviate ovarian failure. As a nonsteroidal aromatase inhibitor, letrozole inhibits the conversion of testosterone to estrogen and subsequently causes PCOS. We divided 6-week-old female mice into the following six groups and evaluated them: vehicle, PCOS, PCOS + MET (metformin), PCOS + DCI, PCOS + EC, and PCOS + DCI + EC. In our study, PCOS rats treated with EC and DCI had low serum LH and T levels and low serum levels of inflammatory cytokines such as TNFα and IL-6. These treatments also appeared to regulate the production of factors that affect follicle formation and inflammation in the ovaries. We concluded that EC extract and/or DCI administration influenced aromatase production and reduced LH and T stimulation, and cotreatment with EC and DCI consequently restored ovarian dysfunction or anti-inflammatory responses in rats with PCOS-like symptoms.
多囊卵巢综合征（PCOS）是一种内分泌状态，其特征为月经稀发、高雄激素血症以及卵巢中存在大量囊性卵泡。在亚洲国家，食用成分和传统药物的使用广为人知，先前的研究表明，[翅藻科]（EC）能够缓解PCOS症状。在类固醇生物合成是关键因素的病症（即PCOS）中给予D-手性肌醇（DCI）已取得了令人满意的结果。因此，我们研究了这两种先前已知的活性化合物的协同作用。在来曲唑诱导的PCOS大鼠中，我们着重研究使用EC和/或DCI提取物的替代疗法来缓解卵巢功能衰竭。作为一种非甾体类芳香化酶抑制剂，来曲唑抑制睾酮向雌激素的转化，进而导致PCOS。我们将6周龄的雌性小鼠分为以下六组并进行评估：溶剂对照组、PCOS组、PCOS + 二甲双胍（MET）组、PCOS + DCI组、PCOS + EC组以及PCOS + DCI + EC组。在我们的研究中，用EC和DCI治疗的PCOS大鼠血清促黄体生成素（LH）和睾酮（T）水平较低，血清中肿瘤坏死因子α（TNFα）和白细胞介素-6（IL-6）等炎性细胞因子水平也较低。这些治疗似乎还能调节影响卵巢卵泡形成和炎症的因子的产生。我们得出结论，EC提取物和/或给予DCI会影响芳香化酶的产生，减少LH和T的刺激，因此，EC和DCI联合治疗可恢复具有PCOS样症状大鼠的卵巢功能障碍或抗炎反应。
