Chambon Christophe, Sayd Thierry, Bourillon Sylvie, Theron Laetitia, Niderkorn Vincent
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), Plateforme d'Exploration du Métabolisme Composante Protéomique (PFEMcp), F-63122 Saint-Genès Champanelle, France.
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), UR 370, Qualité des produits animaux (QuaPA), F-63122 Saint-Genès Champanelle, France.
Data Brief. 2022 Jul 28;43:108492. doi: 10.1016/j.dib.2022.108492. eCollection 2022 Aug.
The protein degradation of alfalfa hay after tannin supplementation was monitored during wethers digestion. Three rumen-cannulated wethers were infused a tannin solution, and water for control, through the cannula. The digestion time-points samples were collected in the rumen and in the abomasum, and the small intestine compartments. The digestomic dataset was acquired by identifying and quantifying the peptides resulting from the protein degradation, using high-resolution LC-MS/MS mass spectrometry and label-free quantitation. The digestomic dataset is the compilation of proteomic data acquired in the rumen and peptidomic data acquired in the abomasum and in the small intestine. The proteomic analysis identified 20 proteins in the rumen fluid, based on 169 peptides of which 140 are unique. The peptidomic analysis identified 28 proteins in the abomasum, based on 575 peptides of which 363 are unique, and 11 proteins in the small intestine, based on 94 peptides of which 63 are unique. This digestomic dataset of proteolysis during sheep post rumen digestion after tannin supplementation reveals the protein regions protected by tannin supplementation, and could be reused in studies related to the protein use efficiency by ruminants.
在阉羊消化过程中监测了添加单宁后苜蓿干草的蛋白质降解情况。三只装有瘤胃瘘管的阉羊通过瘘管分别注入单宁溶液和水作为对照。在瘤胃、皱胃和小肠各部分收集消化时间点的样本。通过使用高分辨率液相色谱-串联质谱法（LC-MS/MS）和无标记定量法，对蛋白质降解产生的肽段进行鉴定和定量，从而获得消化组数据集。该消化组数据集是瘤胃中获得的蛋白质组数据与皱胃和小肠中获得的肽组数据的汇总。蛋白质组分析基于169个肽段（其中140个为独特肽段）在瘤胃液中鉴定出20种蛋白质。肽组分析基于575个肽段（其中363个为独特肽段）在皱胃中鉴定出28种蛋白质，基于94个肽段（其中63个为独特肽段）在小肠中鉴定出11种蛋白质。这个添加单宁后绵羊瘤胃后消化过程中蛋白质水解的消化组数据集揭示了受单宁添加保护的蛋白质区域，可在反刍动物蛋白质利用效率相关研究中重复使用。
