拟南芥 Rab 蛋白 RABC1 通过调节脂滴动态影响气孔发育。
The Arabidopsis Rab protein RABC1 affects stomatal development by regulating lipid droplet dynamics.
机构信息
State Key Laboratory of Hybrid Rice, Department of Plant Sciences, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Hubei Hongshan Laboratory, Wuhan 430070, China.
出版信息
Plant Cell. 2022 Oct 27;34(11):4274-4292. doi: 10.1093/plcell/koac239.
Lipid droplets (LDs) are evolutionarily conserved organelles that serve as hubs of cellular lipid and energy metabolism in virtually all organisms. Mobilization of LDs is important in light-induced stomatal opening. However, whether and how LDs are involved in stomatal development remains unknown. We show here that Arabidopsis thaliana LIPID DROPLETS AND STOMATA 1 (LDS1)/RABC1 (At1g43890) encodes a member of the Rab GTPase family that is involved in regulating LD dynamics and stomatal morphogenesis. The expression of RABC1 is coordinated with the different phases of stomatal development. RABC1 targets to the surface of LDs in response to oleic acid application in a RABC1GEF1-dependent manner. RABC1 physically interacts with SEIPIN2/3, two orthologues of mammalian seipin, which function in the formation of LDs. Disruption of RABC1, RABC1GEF1, or SEIPIN2/3 resulted in aberrantly large LDs, severe defects in guard cell vacuole morphology, and stomatal function. In conclusion, these findings reveal an aspect of LD function and uncover a role for lipid metabolism in stomatal development in plants.
脂滴（LDs）是进化上保守的细胞器，几乎存在于所有生物体中，是细胞脂质和能量代谢的中心。LDs 的动员在光诱导的气孔开放中很重要。然而，LDs 是否以及如何参与气孔发育尚不清楚。我们在这里表明，拟南芥 LIPID DROPLETS AND STOMATA 1（LDS1）/RABC1（At1g43890）编码 Rab GTPase 家族的成员，该家族参与调节 LD 动力学和气孔形态发生。RABC1 的表达与气孔发育的不同阶段相协调。RABC1 响应油酸的应用，以 RABC1GEF1 依赖性的方式靶向到 LD 的表面。RABC1 与 SEIPIN2/3 物理相互作用，SEIPIN2/3 是哺乳动物 seipin 的两个同源物，在 LD 的形成中起作用。RABC1、RABC1GEF1 或 SEIPIN2/3 的破坏导致 LD 异常增大、保卫细胞液泡形态严重缺陷和气孔功能丧失。总之，这些发现揭示了 LD 功能的一个方面，并揭示了脂质代谢在植物气孔发育中的作用。
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