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富化酸奶的抗氧化特性,添加了从药用蘑菇灵芝属中提取的成分。

Antioxidant properties of fortified yogurt with medicinal mushrooms from Phellinus species.

机构信息

Department of Experimental Biochemistry, Institute of Biology, Faculty of Natural Sciences and Mathematics, University Ss. Cyril and Methodius, Skopje, Republic of North Macedonia.

Mycological Laboratory, Department of Botany, Institute of Biology, Faculty of Natural Sciences and Mathematics, University Ss. Cyril and Methodius, Skopje, Republic of North Macedonia.

出版信息

J Food Biochem. 2022 Oct;46(10):e14364. doi: 10.1111/jfbc.14364. Epub 2022 Aug 5.

Abstract

In light of the powerful therapeutic features of Phellinus species and due to the absence of toxic compounds, our investigations were aimed at screening of the antioxidant profile of fortified yogurts with hot water extracts from Phellinus torulosus and Phellinus igniarius leveled to 10%, 5%, and 1% final fortification concentrations after acknowledging their superior bioactive content and radical scavenging capacities (59.77% and 56.73% of DPPH inhibition, respectively) versus cold water extracts (29.87% and 33.18% inhibition rates). Fortified samples signified dose-dependent increases in their inhibition rates during the storage period, with significant differences between 10% fortifications on the 7 day of storage in favor of the samples fortified with P. torulosus. Explicitly, P. torulosus showed 16% higher DPPH and 62.5% higher LPO neutralizing activity than yogurt enriched with P. igniarius. However, prolonged refrigeration tended to equalize antioxidant profiles in both fortified yogurts. Total titratable acidity and pH levels of the fortified yogurts as most important parameters for consumer acceptance were unaltered during storage. PRACTICAL APPLICATIONS: It is a common scientific perception that bioactive compounds present in wild medicinal fungi are the main contributors for their in vitro antioxidant efficiency. On account of these attributes, Phellinus species have been exploited in Far East Asia as safe remedies for many disorders thus making them attractive fortifying ingredients; however, according to our knowledge these mushrooms have never been used as natural additives in beverages. Given the current popularity of yogurt consumption as seen from the global market profits, as well as experimental evidences of enhanced potency of extract in comparison with powder due to maximal bioavailability of antioxidants, it is our belief that this study will increase the interest in the manufacture of functional foods with extracts from wild mushrooms.

摘要

鉴于 Phellinus 属真菌具有强大的治疗特性,且不含有毒化合物,我们的研究旨在筛选添加热水提取物的强化酸奶的抗氧化特性,提取物来自 Phellinus torulosus 和 Phellinus igniarius,最终强化浓度分别为 10%、5%和 1%,前提是它们的生物活性成分和自由基清除能力较高(DPPH 抑制率分别为 59.77%和 56.73%,而冷水提取物的抑制率分别为 29.87%和 33.18%)。强化样品在储存期间的抑制率呈剂量依赖性增加,在储存第 7 天,10%强化水平的差异显著,有利于添加 P. torulosus 的样品。具体来说,P. torulosus 的 DPPH 抑制率比添加 P. igniarius 的酸奶高 16%,LPO 中和活性高 62.5%。然而,长时间冷藏会使两种强化酸奶的抗氧化特性趋于一致。强化酸奶的总可滴定酸度和 pH 值是消费者接受的最重要参数,在储存过程中没有变化。实际应用:人们普遍认为,野生药用真菌中的生物活性化合物是其体外抗氧化效率的主要贡献者。鉴于这些特性,Phellinus 属真菌在远东地区被用作许多疾病的安全治疗方法,因此成为有吸引力的强化成分;然而,据我们所知,这些蘑菇从未作为天然添加剂用于饮料中。考虑到酸奶消费的普及性(从全球市场利润可以看出),以及提取物比粉末具有更高功效的实验证据(因为抗氧化剂的最大生物利用度),我们相信这项研究将增加对用野生蘑菇提取物制造功能性食品的兴趣。

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