Department of Physical Chemistry of Polymers, Max Planck Institute for Polymer Research, Ackermannweg 10, 55128, Mainz, Germany.
Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, P.O. Box 476, 88040-900, Florianópolis-SC, Brazil.
Angew Chem Int Ed Engl. 2022 Sep 26;61(39):e202207998. doi: 10.1002/anie.202207998. Epub 2022 Aug 23.
Understanding the complex behavior and dynamics of cellular membranes is integral to gain insight into cellular division and fusion processes. Bottom-up synthetic cells are as a platform for replicating and probing cellular behavior. Giant polymer vesicles are more robust than liposomal counterparts, as well as having a broad range of chemical functionalities. However, the stability of the membrane can prohibit dynamic processes such as membrane phase separation and division. Here, we present a method for manipulating the membrane of giant polymersomes using a temperature responsive polymer. Upon elevation of temperature deformation and phase separation of the membrane was observed. Upon cooling, the membrane relaxed and became homogeneous again, with infrequent division of the synthetic cells.
理解细胞膜的复杂行为和动力学对于深入了解细胞分裂和融合过程至关重要。自下而上合成的细胞是复制和探测细胞行为的平台。与脂质体相比，巨聚合物囊泡具有更广泛的化学功能，而且更稳定。然而，膜的稳定性可能会阻碍膜相分离和分裂等动态过程。在这里，我们提出了一种使用温度响应聚合物来操纵巨聚合物囊泡膜的方法。升高温度后，观察到膜的变形和相分离。冷却后，膜再次松弛并变得均匀，合成细胞很少分裂。
