伊朗东阿塞拜疆省利用 PCR 技术检测羊奶（山羊）、绵羊和驴奶中的弓形虫 DNA。
Molecular detection of toxoplasma gondii DNA in goats (Capra hircus), sheep (Ovis aries), and donkey (Equus asinus) milk using PCR in East Azerbaijan province, Iran.
机构信息
Department of Food Hygiene and Aquatic, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Department of Food Hygiene and Aquatic, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran; Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
出版信息
Res Vet Sci. 2022 Dec 20;152:58-60. doi: 10.1016/j.rvsc.2022.07.020. Epub 2022 Jul 29.
Toxoplasmosis, a zoonotic infection that is significant for public health (immunocompromised patients, pregnant women) and veterinary medicine (economic losses in the herd), is caused by an intracellular protozoan parasite belonging to the phylum Apicomplexa called Toxoplasma gondii. Consumption of unpasteurized milk and contaminated undercooked meat is a significant source for humans. The present study aimed to determine Toxoplasma gondii DNA in sheep, goats and donkeys Milk kept in East Azerbaijan province using the PCR method based on the B1 gene. For this purpose, 100 milk samples, including 45 sheep, 45 goats and 10 donkeys, were collected from different regions of northwestern Iran using direct milking and then transferred to the Food and Aquatic Health Laboratory under refrigerated conditions. The results showed that out of 100 milk samples examined, 16 samples (16%) were contaminated, and Toxoplasma gondii DNA was detected in 5 (11.11%) sheep, 9 (20%) goats and 2 (20%) donkeys milk specimen, respectively. These findings indicated that Toxoplasma gondii contaminated the raw milk, a human infection source.
弓形虫病是一种人畜共患的传染病，对公共卫生（免疫功能低下的患者、孕妇）和兽医医学（畜群的经济损失）都很重要，它是由一种属于顶复门的细胞内原生动物寄生虫引起的，叫做刚地弓形虫。食用未经巴氏消毒的牛奶和未煮熟的受污染肉类是人类感染的重要来源。本研究旨在使用基于 B1 基因的 PCR 方法检测伊朗西北部东阿塞拜疆省绵羊、山羊和驴奶中的刚地弓形虫 DNA。为此，从伊朗西北部不同地区采集了 100 份牛奶样本，包括 45 份绵羊、45 份山羊和 10 份驴奶，然后在冷藏条件下将其转移到食品和水产卫生实验室。结果表明，在检查的 100 份牛奶样本中，有 16 份（16%）受到污染，在 5 份（11.11%）绵羊、9 份（20%）山羊和 2 份（20%）驴奶样本中检测到刚地弓形虫 DNA。这些发现表明，刚地弓形虫污染了生奶，成为人类感染的来源。
Zotero 插件