School of Life Science and Technology, Harbin Institute of Technology, Harbin 150006, China.
School of Life Science and Technology, Harbin Institute of Technology, Harbin 150006, China; State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, Harbin 150090, China.
Environ Res. 2022 Nov;214(Pt 2):113947. doi: 10.1016/j.envres.2022.113947. Epub 2022 Aug 3.
Pullulan and melanin have become important secondary metabolites that are now widely studied. In this study, a strain of Aureobasidium pullulans HIT-LCY3 was used to ferment potato starch industrial waste to produce pullulan and melanin. After optimization, the culture conditions for the fermentation medium were obtained: inoculum age of 48 h, initial pH of 6.0, inoculation quantity of 1.5%, temperature of 26 °C, fermentation time of 5 d and speed of 160 rpm. Under these conditions, the yield of pullulan was 23.47 g/L with a molecular weight (MW) of 1.21 × 10 Da and the yield of melanin was 18.98 g/L. In addition, the adaptive evolution could significantly increase the yield of pullulan and melanin and the air-floating fermenters was more conductive to product accumulation. Through the 5 L small-scale test and 1000 L pilot test, the yield of pullulan reached 16.52 g/L with molecular weight of 0.92 × 10 Da and the yield of melanin was 12.08 g/L at the trial production of 30,000 L. This work could provide strong support for industrial production and new guidance for waste utilization and environmental protection.
出芽短梗霉多糖和黑色素已成为目前广泛研究的重要次生代谢产物。本研究利用出芽短梗霉 HIT-LCY3 发酵马铃薯淀粉工业废料生产出芽短梗霉多糖和黑色素。经过优化，得到发酵培养基的培养条件：接种龄 48 h、初始 pH 值 6.0、接种量 1.5%、温度 26°C、发酵时间 5 d、转速 160 rpm。在此条件下，出芽短梗霉多糖的产量为 23.47 g/L，分子量（MW）为 1.21×10 Da，黑色素的产量为 18.98 g/L。此外，适应性进化可以显著提高出芽短梗霉多糖和黑色素的产量，气升式发酵罐更有利于产物积累。通过 5 L 小试和 1000 L 中试，在 30000 L 生产试验中，出芽短梗霉多糖的产量达到 16.52 g/L，分子量为 0.92×10 Da，黑色素的产量为 12.08 g/L。本工作可为工业生产提供有力支持，为废物利用和环境保护提供新的指导。
