Minelli G, Franceschini V, Del Grande P, Ciani F
Basic Appl Histochem. 1987;31(1):43-52.
A histochemical light and electron microscopy study of acetylcholinesterase (AChE) was carried out on the regenerating opic tectum of adult newt. A plug of optic tectum was removed and 15 days later [6-H3] thymidine was injected. Ninety days after the lesion the brain was removed, treated for histochemical AChE-detection and autoradiographic analysis. This double treatment showed the capacity of these adult amphibians to regenerate the nervous tissue through the proliferation of undifferentiated elements and their subsequent differentiation into neurons as is shown by the presence of cells both labelled by [6-H3] thymidine and by the AChE-reaction product.
对成年蝾螈再生视顶盖进行了乙酰胆碱酯酶（AChE）的组织化学光镜和电镜研究。切除一块视顶盖，15天后注射[6-H3]胸腺嘧啶核苷。损伤90天后取出大脑，进行组织化学AChE检测和放射自显影分析。这种双重处理表明，这些成年两栖动物能够通过未分化成分的增殖以及随后分化为神经元来再生神经组织，这一点通过既被[6-H3]胸腺嘧啶核苷标记又有AChE反应产物的细胞的存在得以体现。
