Danova M, Mazzini G, Wilson G, Ucci G, Dionigi P, Riccardi A, Fiocca R
Basic Appl Histochem. 1987;31(1):73-82.
Flow cytometric DNA analysis was performed on both fresh and on paraffin embedded samples obtained by gastroscopic biopsies in 5 patients with histologically normal gastric mucosa (20 specimens) and by radical gastrectomies in 9 cases of human gastric cancer (36 specimens). Ploidy and the distribution of cells in the different cell cycle phases were estimated. Results from fresh tissue were compared with those from paraffin embedded material. All the samples of normal mucosa showed a diploid modal DNA content. Unimodal DNA distribution was found both in fresh and paraffin embedded material of 3 well differentiated gastric adenocarcinomas, whereas the remaining 6 tumors (1 moderately differentiated, 4 poorly differentiated and 1 undifferentiated adenocarcinomas) showed aneuploidy. The percentage of cells in S phase in normal tissue and in tumors were respectively 9.0% and 11.9% in the fresh material, and 10.0% and 15.0% in the paraffin embedded material. No statistically significant differences were found between fresh and paraffin embedded samples, whereas the proliferative activity was, in both cases, statistically higher in tumors than in normal mucosa (p less than .01 and p less than .001 respectively). The quality of DNA flow cytometry from paraffin embedded material was comparable with that from fresh samples.
对5例组织学正常胃黏膜患者经胃镜活检获取的新鲜及石蜡包埋样本（20份标本）以及9例人胃癌患者经根治性胃切除获取的样本（36份标本）进行了流式细胞术DNA分析。估计了倍体情况以及不同细胞周期阶段细胞的分布。将新鲜组织的结果与石蜡包埋材料的结果进行了比较。所有正常黏膜样本均显示二倍体模式DNA含量。在3例高分化胃腺癌的新鲜及石蜡包埋材料中均发现单峰DNA分布，而其余6例肿瘤（1例中分化、4例低分化和1例未分化腺癌）显示非整倍体。新鲜材料中正常组织和肿瘤组织S期细胞百分比分别为9.0%和11.9%，石蜡包埋材料中分别为10.0%和15.0%。新鲜样本与石蜡包埋样本之间未发现统计学上的显著差异，然而，在这两种情况下，肿瘤组织的增殖活性在统计学上均高于正常黏膜（分别为p<0.01和p<0.001）。石蜡包埋材料的DNA流式细胞术质量与新鲜样本相当。
