Oshiro T, Baba H, Okuyama T, Inutsuka S, Takeuchi H, Adachi Y, Maehara Y, Sugimachi K
Dept. of Surgery II, Faculty of Medicine, Kyushu University.
Gan To Kagaku Ryoho. 1993 Apr;20(6):709-12.
Histopathologic studies and fluorocytometric DNA analysis were carried out in 39 patients with intestinal metaplasia (IM) of the mucosa associated with gastric carcinoma, in order to determine possible relationships. According to the histological degree of IM, 39 cases were divided into 16 (41%) with slight-to-moderate, and 23 (59%) with severe IM. The 39 tumors were histologically divided into 20 (51%) differentiated tumors and 19 (49%) undifferentiated tumors. The DNA patterns of tumors revealed 17 (43%) to be euploidy and 22 (57%) to be aneuploidy. The DNA content of the metaplastic epithelial cells was measured, and the percentage of the cells in S and G2 + M phases of the cell cycle was taken as the index of proliferative activity. DNA analysis of the non-metaplastic epithelium was performed in 10 as a control group. While the histological differentiation of the tumors and the degree of IM were not related to the proliferative activity of IM, the IM around the aneuploid tumors did have higher proliferative activity (14.01 +/- 6.16%) than that around euploid tumors (11.08 +/- 7.13%) or of normal mucosa (9.95 +/- 9.92%). This approach will help to determine cell kinetics of IM around the gastric tumor. Our study indicated a relationship between DNA ploidy and the proliferative activity of IM around gastric cancer.
对39例与胃癌相关的黏膜肠化生（IM）患者进行了组织病理学研究和荧光细胞DNA分析，以确定可能的关系。根据IM的组织学程度，39例患者分为轻度至中度IM 16例（41%）和重度IM 23例（59%）。39个肿瘤在组织学上分为分化型肿瘤20例（51%）和未分化型肿瘤19例（49%）。肿瘤的DNA模式显示17例（43%）为整倍体，22例（57%）为非整倍体。测量化生上皮细胞的DNA含量，并将细胞周期中S期和G2+M期细胞的百分比作为增殖活性指标。选取10例作为对照组，对非化生上皮进行DNA分析。虽然肿瘤的组织学分化和IM程度与IM的增殖活性无关，但非整倍体肿瘤周围的IM增殖活性（14.01±6.16%）高于整倍体肿瘤周围的IM（11.08±7.13%）或正常黏膜（9.95±9.92%）。这种方法将有助于确定胃肿瘤周围IM的细胞动力学。我们的研究表明DNA倍性与胃癌周围IM的增殖活性之间存在关联。
