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人红细胞膜中磷酸烯醇丙酮酸和无机磷酸的主动运输中心。

The active center of transport for phosphoenolpyruvate and inorganic phosphate in the human erythrocyte membrane.

作者信息

Hamasaki N, Kawano Y, Inoue H

出版信息

Biomed Biochim Acta. 1987;46(2-3):S51-4.

PMID:3593317
Abstract

Phosphoenolpyruvate is transported into erythrocytes by a carrier-mediated transport system. The transport is competitively inhibited by inorganic phosphate and pyridoxal phosphate, which are transported across the cell membrane by Band 3. The pH profiles for the transport of inorganic phosphate and phosphoenolpyruvate suggest that a histidine residue exposed at the inner surface of the membrane participates in both of the transport. A hydrophobic peptide which may be a part of the active center for the transport is purified from the CNBr fragments of Band 3.

摘要

磷酸烯醇丙酮酸通过载体介导的转运系统进入红细胞。该转运受到无机磷酸盐和磷酸吡哆醛的竞争性抑制,它们通过带3跨细胞膜转运。无机磷酸盐和磷酸烯醇丙酮酸转运的pH曲线表明,膜内表面暴露的组氨酸残基参与了这两种转运。从带3的溴化氰片段中纯化出一种可能是转运活性中心一部分的疏水肽。

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