Hamasaki N, Okubo K
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Faculty of Medicine, Fukuoka, Japan.
Cell Mol Biol (Noisy-le-grand). 1996 Nov;42(7):1025-39.
Band 3 protein is a typical polytropic membrane protein and mediates the exchange of the cellular HCO3- with CI- in plasma, which has been known as the "Chloride Shift". Owing to the "Chloride Shift", red blood cells can discriminate the metabolically active cells from inactive cells and deliver oxygen particularly to metabolically active tissues that produce carbon dioxide. Thus, band 3 protein is a sensor for metabolically active tissues and no excess oxygen is supplied to tissues as far as oxygen is delivered by red blood cells. In this chapter, we review the physiological role of the anion exchange mediated by band 3 protein and our work concerning the structure and function relationship in band 3 protein, that, is, affinity labeling of the active center for the anion exchange with pyridoxal phosphate, conformational change during the anion exchange process, examination of fidelity of hydropathy prediction on band 3 protein, and phosphoenolpyruvate transport mediated by band 3 protein and its clinical application.
带3蛋白是一种典型的多向性膜蛋白，介导细胞内的HCO3-与血浆中的Cl-进行交换，这一过程被称为“氯转移”。由于“氯转移”，红细胞能够区分代谢活跃细胞和非活跃细胞，并将氧气特别输送到产生二氧化碳的代谢活跃组织。因此，带3蛋白是代谢活跃组织的一种传感器，只要氧气是由红细胞输送的，就不会向组织供应过量的氧气。在本章中，我们将综述带3蛋白介导的阴离子交换的生理作用，以及我们关于带3蛋白结构与功能关系的研究工作，即与磷酸吡哆醛对阴离子交换活性中心的亲和标记、阴离子交换过程中的构象变化、对带3蛋白亲水性预测准确性的检验，以及带3蛋白介导的磷酸烯醇丙酮酸转运及其临床应用。
