Smith B D, La Celle P L
Biomed Biochim Acta. 1987;46(2-3):S93-7.
Adherence of normal human erythrocytes to cultured endothelial cells when exposed to in vitro fluid shear forces typical of venules, capillaries and arterioles is very low, less than 0.03 percent, in the presence of an albumin-buffer but increases five fold by the effects of autologous plasma. Senescent erythrocytes obtained by density separation had an adherence frequency in the normal cell range, suggesting that predisposition to endothelial adherence is not a key factor in regulation of life span by the sequestration, identification and phagocytosis modulated by immune recognition. Sickle cells' frequency of adherence exceeds that of normal cells particularly at low shear forces calculated to resemble those of veins and sinuses, and increases five to ten fold when autologous plasma obtained during sickle crisis is present; these findings add support to the hypothesis that excessive tendency for sickle cell adherence may contribute to the vascular obstructive phenomena of the disorder. The behavior of hereditary spherocytes and thalassemic erythrocytes resembles normal cells, suggesting that hemolytic mechanisms are not significantly dependent on erythrocyte-endothelial cell interaction. Erythrocytes from patients having diabetes exhibit the characteristics of control cells, suggesting that their membrane adhesion to endothelium may not be critical to initiation of vessel wall pathophysiological processes; however, the behavior in relation to damaged endothelial cells may differ importantly.
当暴露于体外小静脉、毛细血管和小动脉典型的流体剪切力时，在存在白蛋白缓冲液的情况下，正常人红细胞与培养的内皮细胞的黏附率非常低，低于0.03%，但在自体血浆的作用下会增加五倍。通过密度分离获得的衰老红细胞的黏附频率在正常细胞范围内，这表明内皮黏附的易感性不是通过免疫识别调节的隔离、识别和吞噬作用来调节寿命的关键因素。镰状细胞的黏附频率超过正常细胞，特别是在计算得出类似于静脉和静脉窦的低剪切力下，并且当存在镰状危机期间获得的自体血浆时会增加五到十倍；这些发现支持了镰状细胞过度黏附倾向可能导致该疾病血管阻塞现象的假说。遗传性球形红细胞和地中海贫血红细胞的行为类似于正常细胞，这表明溶血机制并不显著依赖于红细胞与内皮细胞的相互作用。糖尿病患者的红细胞表现出对照细胞的特征，这表明它们与内皮的膜黏附可能对血管壁病理生理过程的启动并不关键；然而，它们与受损内皮细胞相关的行为可能有重要差异。
