对甲壳类动物肌肉进行的体内31P核磁共振研究：秀丽长臂虾尾部肌肉的疲劳与恢复

In vivo 31P NMR in crustacean muscles: fatigue and recovery in the tail musculature from the prawn Palaemon elegans.

作者信息

Thébault M T, Raffin J P, Le Gall J Y

出版信息

Biochem Biophys Res Commun. 1987 May 29;145(1):453-9. doi: 10.1016/0006-291x(87)91342-8.

Abstract

31P NMR has been used to observe the in vivo phosphometabolite concentrations in the tail musculature from the prawn Palaemon elegans, at rest and after escape swimming and subsequent recovery. Muscular fatigue corresponds to a 60% breakdown of phosphoarginine, and a 45% increase of sugar phosphates. The pHi fell from 7.10 to 6.86. During recovery, the sugar phosphates and arginine phosphate are replenished after 20 minutes. The ATP concentration did not change throughout the experiment. The pHi was restored within 20 minutes.

摘要

31P核磁共振已被用于观察秀丽长臂虾尾部肌肉组织在静息状态、逃避游泳及随后恢复过程中的体内磷酸代谢物浓度。肌肉疲劳对应着磷酸精氨酸60%的分解以及糖磷酸酯45%的增加。细胞内pH值从7.10降至6.86。在恢复过程中，糖磷酸酯和磷酸精氨酸在20分钟后得到补充。整个实验过程中ATP浓度没有变化。细胞内pH值在20分钟内恢复。