Koshihara Y, Kawamura M, Oda H, Higaki S
Biochem Biophys Res Commun. 1987 Jun 15;145(2):651-7. doi: 10.1016/0006-291x(87)91014-x.
Human osteoblastic cell cultures were established from human periosteum, identified on the basis of high alkaline phosphatase activity in the confluent state. The cells in the growing state showed a fibroblastic morphology and grew to form multiple layers. The cells could spontaneously calcify, and the process was accelerated by alpha-glycerophosphate. Minerals deposited on the cells consisted exclusively of calcium and phosphorus, and matured into hydroxyapatite crystals. The calcification was stimulated by the treatment with 1 alpha,25-dihydroxy-vitamin D3. These results indicate that the osteoblastic cells have the capacity to differentiate into osteocytes and form calcified human bone tissue in vitro.
人成骨细胞培养物是从人骨膜中建立的，根据汇合状态下的高碱性磷酸酶活性进行鉴定。生长状态的细胞呈成纤维细胞形态，生长形成多层。细胞可自发钙化，α-甘油磷酸可加速这一过程。沉积在细胞上的矿物质仅由钙和磷组成，并成熟为羟基磷灰石晶体。用1α,25-二羟基维生素D3处理可刺激钙化。这些结果表明，成骨细胞具有在体外分化为骨细胞并形成钙化人骨组织的能力。
