Koshihara Y, Hirano M, Kawamura M, Oda H, Higaki S
Department of Pharmacology, Tokyo Metropolitan Institute of Gerontology.
J Gerontol. 1991 Sep;46(5):B201-6. doi: 10.1093/geronj/46.5.b201.
Studies of the mechanisms of osteoporosis have not yet determined whether these conditions result from increased osteoclast activity or decreased osteoblast activity, or perhaps both. Osteoporosis is related to aging and to postmenopausal status. The function and the mitotic capacity of cultured human osteoblast-like cells were investigated in this study. The age at which these cells lose the ability to divide showed a strong negative correlation with donor age (r = .815, p less than .01). There was also significant correlation of maximum cell saturation density with donor age (r = .698, p less than .01). Alkaline phosphatase (ALP) activity and mineralization ability, typical functions of osteoblasts, continue undiminished up to the point at which mitotic capacity ceases. When cells were treated with 1 alpha, 25-dihydroxy vitamin D3 in the presence of 2 mM alpha-glycerophosphate, ALP activity and mineralization ability showed an increase, rather than a decrease, with advancing donor age. However, these functions showed no correlation with in vitro cell aging. We propose that human osteoblast-like cells from elderly subjects do not lose their functions of mineralization and ALP formation, and that loss of these functions with cell aging does not correlate with advancing age.
对骨质疏松症发病机制的研究尚未确定这些情况是由破骨细胞活性增加还是成骨细胞活性降低，或者可能两者兼而有之所致。骨质疏松症与衰老及绝经后状态有关。本研究对培养的人成骨样细胞的功能及有丝分裂能力进行了研究。这些细胞失去分裂能力的年龄与供体年龄呈显著负相关（r = 0.815，p < 0.01）。最大细胞饱和密度与供体年龄也存在显著相关性（r = 0.698，p < 0.01）。碱性磷酸酶（ALP）活性和矿化能力是成骨细胞的典型功能，在有丝分裂能力停止之前一直保持不变。当细胞在2 mM α-甘油磷酸存在的情况下用1α，25-二羟基维生素D3处理时，随着供体年龄的增加，ALP活性和矿化能力呈增加而非降低趋势。然而，这些功能与体外细胞衰老无关。我们认为，老年受试者的人成骨样细胞不会丧失其矿化和ALP形成功能，并且这些功能随细胞衰老而丧失与年龄增长无关。
