Department of Chemistry, Catalysts and Organic Synthesis Research Laboratory, Iran University of Science and Technology, Tehran 16846-13114, Iran.
Department of Medical Biotechnology, Protein Chemistry Laboratory, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran; Department of Chemistry, Advanced Chemistry Studies Lab, K. N. Toosi University of Technology, Tehran, Iran.
J Chromatogr A. 2022 Aug 30;1679:463376. doi: 10.1016/j.chroma.2022.463376. Epub 2022 Jul 25.
In this study, a novel and green chitosan-metformin/NiCl/FeO nanobiocomposite was synthesized and used to purify alkaline phosphatase (ALPs) from hen's egg yolk. For this purpose, after functionalization of the chitosan biopolymer by terephthaloyl chloride-metformin ligand, the coordination with Ni(II) and magnetization process were performed. The structure and properties of the synthesized nanobiocomposite were then evaluated by using analyzes such as FT-IR, EDX, FE-SEM, XRD, TGA and VSM. Purification of ALPs with chitosan-metformin/NiCl/FeO nanobiocomposite is a fast, reusable and cost-effective method. By this protocol, 62% purification efficiency was obtained and the synthesized nanobiocomposite was not attached to other proteins in hen's egg yolk. ALPs was obtained approximately in the pure form and the purification process was evaluated using SDS-PAGE. The reusability of nanobiocomposites was evaluated and a slight decrease in adsorption capacity was observed after 4 cycles.
在这项研究中，合成了一种新型绿色壳聚糖-二甲双胍/NiCl/FeO 纳米生物复合材料，并将其用于从鸡蛋黄中纯化碱性磷酸酶（ALPs）。为此，壳聚糖生物聚合物首先通过对苯二甲酰氯-二甲双胍配体进行功能化，然后与 Ni(II)配位并进行磁化过程。通过 FT-IR、EDX、FE-SEM、XRD、TGA 和 VSM 等分析方法对合成的纳米生物复合材料的结构和性能进行了评估。壳聚糖-二甲双胍/NiCl/FeO 纳米生物复合材料用于 ALPs 的纯化是一种快速、可重复使用且具有成本效益的方法。通过该方案，获得了 62%的纯化效率，并且合成的纳米生物复合材料未与鸡蛋黄中的其他蛋白质结合。ALPs 以近乎纯的形式获得，并使用 SDS-PAGE 评估了纯化过程。评估了纳米生物复合材料的可重复使用性，在 4 个循环后观察到吸附容量略有下降。
