Eivazzadeh-Keihan Reza, Pajoum Zeinab, Aliabadi Hooman Aghamirza Moghim, Mohammadi Adibeh, Kashtiaray Amir, Bani Milad Salimi, Pishva Banafshe, Maleki Ali, Heravi Majid M, Mahdavi Mohammad, Ziaei Ziabari Elaheh
Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology Tehran 16846-13114 Iran
Department of Chemistry, School of Physics and Chemistry, Alzahra University PO Box 1993891176, Vanak Tehran Iran
RSC Adv. 2023 Mar 14;13(13):8540-8550. doi: 10.1039/d3ra00612c.
Herein, a multifunctional nanobiocomposite was designed for biological application, amongst which hyperthermia cancer therapy application was specifically investigated. This nanobiocomposite was fabricated based on chitosan hydrogel (CS), silk fibroin (SF), water-soluble polymer polyvinyl alcohol (PVA) and iron oxide magnetic nanoparticles (FeO MNPs). CS and SF as natural compounds were used to improve the biocompatibility, biodegradability, adhesion and cell growth properties of the nanobiocomposite that can prepare this nanocomposite for the other biological applications such as wound healing and tissue engineering. Since the mechanical properties are very important in biological applications, PVA polymer was used to increase the mechanical properties of the prepared nanobiocomposite. All components of this nanobiocomposite have good dispersion in water due to the presence of hydrophilic groups such as NH, OH, and COOH, which is one of the effective factors in increasing the efficiency of hyperthermia cancer therapy. The structural analyzes of the hybrid nanobiocomposite were determined by FT-IR, XRD, EDX, FE-SEM, TGA and VSM. Biological studies such as MTT and hemolysis testing proved that it is hemocompatible and non-toxic for healthy cells. Furthermore, it can cause the death of cancer cells to some extent (20.23%). The ability of the nanobiocomposites in hyperthermia cancer therapy was evaluated. Also, the results showed that it can be introduced as an excellent candidate for hyperthermia cancer therapy.
在此，设计了一种用于生物应用的多功能纳米生物复合材料，其中特别研究了其在热疗癌症治疗中的应用。这种纳米生物复合材料是基于壳聚糖水凝胶（CS）、丝素蛋白（SF）、水溶性聚合物聚乙烯醇（PVA）和氧化铁磁性纳米颗粒（FeO MNPs）制备的。CS和SF作为天然化合物，用于改善纳米生物复合材料的生物相容性、生物降解性、粘附性和细胞生长特性，从而为伤口愈合和组织工程等其他生物应用制备这种纳米复合材料。由于机械性能在生物应用中非常重要，因此使用PVA聚合物来提高所制备纳米生物复合材料的机械性能。由于存在诸如NH、OH和COOH等亲水基团，该纳米生物复合材料的所有组分在水中具有良好的分散性，这是提高热疗癌症治疗效率的有效因素之一。通过傅里叶变换红外光谱（FT-IR）、X射线衍射（XRD）、能量色散X射线光谱（EDX）、场发射扫描电子显微镜（FE-SEM）、热重分析（TGA）和振动样品磁强计（VSM）对杂化纳米生物复合材料进行了结构分析。MTT和溶血试验等生物学研究证明，它对健康细胞具有血液相容性且无毒。此外，它在一定程度上（20.23%）可导致癌细胞死亡。评估了纳米生物复合材料在热疗癌症治疗中的能力。结果还表明，它可被视为热疗癌症治疗的优秀候选材料。
