Yoshimoto T, Mihama T, Takahashi K, Saito Y, Tamaura Y, Inada Y
Biochem Biophys Res Commun. 1987 Jun 15;145(2):908-14. doi: 10.1016/0006-291x(87)91051-5.
An activated magnetic modifier, which could render biological materials magnetic property, was synthesized in following two steps: oxidation of ferrous ions (Fe2+) with hydrogen peroxide in the presence of alpha, omega-dicarboxymethylpoly(oxyethylene) (DCPEG) to obtain DCPEG-magnetite (Fe3O4); free carboxyl groups in the DCPEG-magnetite were activated with N-hydroxysuccinimide. By coupling the activated magnetic modifier to amino groups of lipase or L-asparaginase, magnetic enzymes were prepared. They dispersed stably not only in aqueous solution but also in organic solvents with high enzymic activities. Magnetic enzymes were readily recovered from reaction mixture in a magnetic field of 6000 Oe without loss of enzymic activity.
一种能够赋予生物材料磁性的活性磁性修饰剂，通过以下两步合成：在α,ω-二羧甲基聚（氧乙烯）（DCPEG）存在下，用过氧化氢氧化亚铁离子（Fe2+）以获得DCPEG-磁铁矿（Fe3O4）；用N-羟基琥珀酰亚胺活化DCPEG-磁铁矿中的游离羧基。通过将活性磁性修饰剂与脂肪酶或L-天冬酰胺酶的氨基偶联，制备了磁性酶。它们不仅能稳定地分散在水溶液中，还能分散在具有高酶活性的有机溶剂中。在6000奥斯特的磁场中，磁性酶很容易从反应混合物中回收，且酶活性不会丧失。
