具有磁性的尿激酶介导的纤维蛋白溶解作用。

Fibrinolysis by urokinase endowed with magnetic property.

作者信息

Inada Y, Ohwada K, Yoshimoto T, Kojima S, Takahashi K, Kodera Y, Matsushima A, Saito Y

机构信息

Laboratory of Biological Chemistry, Tokyo Institute of Technology, Japan.

出版信息

Biochem Biophys Res Commun. 1987 Oct 14;148(1):392-6. doi: 10.1016/0006-291x(87)91123-5.

DOI:10.1016/0006-291x(87)91123-5

PMID:3675586

Abstract

The activated magnetic modifier was synthesized from magnetite, alpha, omega-dicarboxymethylpoly(oxyethylene) and N-hydroxysuccinimide (Biochem. Biophys. Res. Commun., 145, 908-914, 1987). Urokinase was directly coupled with the activated magnetic modifier to obtain magnetic urokinase. The magnetic urokinase dispersed in saline and exerted high fibrinolytic activity (13.8 X 10(4) IU/mg protein), and was readily recovered from saline by magnetic force of 250 Oe. By applying magnetic force, the urokinase was attracted at our will and local fibrinolysis was achieved on fibrin gel in a petri dish.

摘要

活性磁性修饰剂由磁铁矿、α,ω-二羧甲基聚(氧乙烯)和N-羟基琥珀酰亚胺合成(《生物化学与生物物理研究通讯》，145卷，908 - 914页，1987年)。将尿激酶直接与活性磁性修饰剂偶联以获得磁性尿激酶。磁性尿激酶分散在盐水中并表现出高纤溶活性(13.8×10⁴IU/mg蛋白质)，并且通过250奥斯特的磁力很容易从盐水中回收。通过施加磁力，尿激酶可按我们的意愿被吸引，从而在培养皿中的纤维蛋白凝胶上实现局部纤溶。