Nakano M, Hatanaka Y, Sawai T, Kobayashi N, Tobita T
Biochem Int. 1987 Mar;14(3):417-23.
The glycoproteins of porcine zonae pellucidae were fractionated into three families (PZP1-3) by high-performance liquid chromatography on a gel filtration column. Their molecular weights were estimated to be 164K for PZP1, 99K for PZP2, and 55K for PZP3; and they were present in an approximate weight ratio of protein moiety of 2:3:18, respectively. Differences in carbohydrate composition position between them were found, although their amino acid compositions were similar to each other. CD spectra showed that the three families had an almost identical secondary structure of the helical form in the presence of SDS. In PBS without SDS, they had different secondary structures, predominantly in the beta-form, and probably different tertiary structures.
通过在凝胶过滤柱上进行高效液相色谱，将猪透明带糖蛋白分为三个家族（PZP1 - 3）。它们的分子量估计分别为：PZP1为164K，PZP2为99K，PZP3为55K；且它们蛋白质部分的重量比约为2:3:18。尽管它们的氨基酸组成彼此相似，但发现它们之间碳水化合物组成位置存在差异。圆二色谱显示，在十二烷基硫酸钠（SDS）存在的情况下，这三个家族具有几乎相同的螺旋形式二级结构。在没有SDS的磷酸盐缓冲盐水（PBS）中，它们具有不同的二级结构，主要为β-形式，并且可能具有不同的三级结构。
