胰蛋白酶和链霉蛋白酶对猪透明带的溶解作用。

Solubilization of porcine zonae pellucidae by trypsin and pronase.

作者信息

Nakano M, Hatanaka Y, Tobita T

出版信息

Biochem Int. 1987 Mar;14(3):425-33.

Abstract

The porcine zona pellucida was dissolved with difficulty by trypsin in isotonic solution, whereas it was efficiently dissolved by pronase. A structural change of the zona was induced in hypotonic solution, resulting in acceleration of dissolution by these proteases. The solubilization rate of three families (PZP1-3) of zona protein by both enzymes was analyzed by HPLC. In hypotonic solution, PZP1 was solubilized first, followed by PZP2; and PZP3 was then finally released. In isotonic solution, PZP1 and PZP2 were also solubilized faster than PZP3, which was almost completely resistant to trypsin, showing that the solubilization of the zona depended on that of PZP3. Noticeably, high molecular weight products were produced as the proteolytic hydrolysis proceeded in PBS. Circular dichroic spectra and electrophoretograms of the tryptic hydrolysates showed that the zona may have a regular supramolecular structure.

摘要

猪透明带在等渗溶液中很难被胰蛋白酶溶解，而链霉蛋白酶能有效溶解它。在低渗溶液中透明带会发生结构变化，导致这些蛋白酶加速溶解。通过高效液相色谱分析了两种酶对透明带蛋白三个家族（PZP1 - 3）的溶解率。在低渗溶液中，PZP1首先被溶解，接着是PZP2；然后PZP3最终被释放。在等渗溶液中，PZP1和PZP2的溶解速度也比PZP3快，PZP3几乎完全抵抗胰蛋白酶，这表明透明带的溶解取决于PZP3。值得注意的是，在磷酸盐缓冲盐溶液中进行蛋白水解时会产生高分子量产物。胰蛋白酶水解产物的圆二色光谱和电泳图表明，透明带可能具有规则的超分子结构。