CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Centre for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China.
School of Pharmacy, Binzhou Medical University, Yantai 264003, China.
J Hazard Mater. 2022 Oct 15;440:129711. doi: 10.1016/j.jhazmat.2022.129711. Epub 2022 Aug 3.
Microplastics (MPs) have the characteristics of large specific surface area, high hydrophobicity and surface charge, so they are easy to combine with other pollutants and cause toxic effects on aquatic organisms. Here, we prepared a polyvinyl chloride-microplastics (PVC-MPs) fragmentation model to simulate the real microplastic state, and characterized its composition, morphology, particle size and zeta potential. On this basis, we used single and compound exposure of PVC and di(2-ethylhexyl) phthalate (DEHP) to explore their effects on hatchability and mortality of zebrafish (Danio rerio) embryos and toxicity to oxidative stress and cardiac development in zebrafish larvae. Herein, PVC-MPs slowed down the hatching rate of zebrafish embryos and induced the death of zebrafish, while DEHP could slow down the induced of death, it had no effect on hatching rate. The PVC-MPs/DEHP single pollution could induce the reactive oxygen species (ROS) and activated the antioxidant defense signaling pathway, while the compound group showed the level of feedback autoregulation of NF-E2-related factor 2 (Nrf2) signaling pathway. The single pollution also could inhibit the expression of genes related to cardiac development, while the combined pollution showed an antagonistic effect. This study provided a theoretical basis for the ecotoxicology and biomonitoring of MPs in the natural state.
微塑料（MPs）具有比表面积大、疏水性和表面电荷高等特点，容易与其他污染物结合，对水生生物产生毒性作用。在这里，我们制备了聚氯乙烯-微塑料（PVC-MPs）破碎模型来模拟真实的微塑料状态，并对其组成、形貌、粒径和zeta 电位进行了表征。在此基础上，我们采用 PVC 和邻苯二甲酸二（2-乙基己基）酯（DEHP）的单一和复合暴露，来探讨它们对斑马鱼（Danio rerio）胚胎孵化率和死亡率的影响以及对斑马鱼幼虫氧化应激和心脏发育的毒性。结果表明，PVC-MPs 降低了斑马鱼胚胎的孵化率并诱导其死亡，而 DEHP 则可以减缓诱导死亡的速度，但对孵化率没有影响。PVC-MPs/DEHP 单一污染可诱导活性氧（ROS）产生并激活抗氧化防御信号通路，而复合组则表现出 NF-E2 相关因子 2（Nrf2）信号通路的反馈自动调节水平。单一污染还可抑制与心脏发育相关基因的表达，而复合污染则表现出拮抗作用。本研究为 MPs 在自然状态下的生态毒理学和生物监测提供了理论依据。
