Department of Biology, University of Naples Federico II, 80126 Naples, Italy.
Department of Sciences and Technologies, University of Sannio, 82100 Benevento, Italy.
Int J Mol Sci. 2024 Jun 27;25(13):7063. doi: 10.3390/ijms25137063.
In this study, we present data on the effects of condensed tannins (CTs) and hydrolysable tannins (HTs), polyphenols extracted from plants, at different concentrations on zebrafish development to identify the range of concentrations with toxic effects. Zebrafish embryos were exposed to CTs and HTs at two different concentration ranges (5.0-20.0 μgL and 5.0-20.0 mgL) for 72 h. The toxicity parameters were observed up to 72 h of treatment. The uptake of CTs and HTs by the zebrafish larvae was assessed via HPLC analysis. A qRT-PCR analysis was performed to evaluate the expressions of genes , , and , involved in the hatching process of zebrafish. CTs and HTs at 5.0, 10.0, and 20.0 μgL were not toxic. On the contrary, at 5.0, 10.0, and 20.0 mgL, HTs induced a delay in hatching starting from 48 h of treatment, while CTs showed a delay in hatching mainly at 48 h. The analysis of gene expression showed a downregulation in the group exposed to HTs, confirming the hatching data. We believe that this study is important for defining the optimal doses of CTs and HTs to be employed in different application fields such as the chemical industry, the animal feed industry, and medical science.
在这项研究中，我们展示了植物来源的缩合单宁（CTs）和可水解单宁（HTs）、多酚在不同浓度下对斑马鱼发育的影响数据，以确定具有毒性作用的浓度范围。将斑马鱼胚胎暴露于 CTs 和 HTs 两个不同浓度范围（5.0-20.0 μgL 和 5.0-20.0 mgL）72 小时。在处理 72 小时内观察毒性参数。通过 HPLC 分析评估 CTs 和 HTs 在斑马鱼幼虫中的摄取情况。进行 qRT-PCR 分析以评估参与斑马鱼孵化过程的基因 、 、 和 的表达。5.0、10.0 和 20.0 μgL 的 CTs 和 HTs 没有毒性。相反，在 5.0、10.0 和 20.0 mgL 时，HTs 从处理 48 小时开始诱导孵化延迟，而 CTs 主要在 48 小时时显示孵化延迟。基因表达分析显示暴露于 HTs 的组下调，证实了孵化数据。我们认为这项研究对于定义 CTs 和 HTs 的最佳剂量在不同应用领域（如化工、动物饲料工业和医学科学）中具有重要意义。
