School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan 453003, PR China.
School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan 453003, PR China.
Int J Biol Macromol. 2022 Oct 31;219:346-352. doi: 10.1016/j.ijbiomac.2022.07.251. Epub 2022 Aug 4.
As a toxic substance on human health produced in food thermal treatment, simple analytical approaches are highly desired for the detection of acrylamide (ACR) in foods. With the aid of exonuclease III (Exo III), a simple fluorescence sensor was proposed based on carboxyfluorescein-labeled double-stranded DNA (FAM-dsDNA) and a cationic conjugated polymer (PFP). Fluorescence resonance energy transfer (FRET) efficiency between FAM and PFP was changed with and without ACR. When ACR was present, ACR and single-stranded DNA (P1, ssDNA) formed an adduct, allowing free FAM-labeled complementarity strand DNA (P2, FAM-csDNA) to appear in the solution and avoiding the digestion of P2 by Exo III. After the addition of PFP, the interaction of PFP and FAM induced strong FRET. Under optimized conditions, ACR was detected with a limit of detection (LOD) of 0.16 μM. According to this biosensor, a LOD of 1.3 μM in water extract samples was observed with a good recovery rate (95-110 %).
作为食品热加工过程中对人体健康产生的有毒物质，人们非常希望能够采用简单的分析方法来检测食品中的丙烯酰胺（ACR）。本研究借助核酸外切酶 III（Exo III），基于羧基荧光素标记的双链 DNA（FAM-dsDNA）和阳离子共轭聚合物（PFP）构建了一种简单的荧光传感器。有无 ACR 存在时，FAM 和 PFP 之间的荧光共振能量转移（FRET）效率会发生变化。当存在 ACR 时，ACR 和单链 DNA（P1，ssDNA）形成加合物，使得溶液中出现游离的 FAM 标记互补链 DNA（P2，FAM-csDNA），从而避免 P2 被 Exo III 消化。加入 PFP 后，PFP 和 FAM 的相互作用会引发强烈的 FRET。在优化条件下，该传感器对 ACR 的检测限（LOD）为 0.16 μM。根据该生物传感器，在水提取物样品中的 LOD 为 1.3 μM，回收率良好（95-110%）。
