MacKinnon S K, Starkebaum G
Arthritis Rheum. 1987 May;30(5):498-506. doi: 10.1002/art.1780300503.
Monocytes from 11 patients with rheumatoid arthritis and 10 control subjects were purified by countercurrent elutriation. Rheumatoid arthritis monocytes had more cell-associated IgG (P less than 0.001) and bound more 125I-labeled heat-aggregated IgG in vitro (P less than 0.02) than did monocytes from control subjects. Interaction of rheumatoid factor (RF) with monocytes was then investigated. Purified 125I-labeled IgM-RF and IgG-RF bound directly to monocytes from normal individuals. Furthermore, preincubation of normal monocytes with RF augmented subsequent binding of aggregated IgG to the cells. We conclude that monocyte-associated RF can enhance binding of IgG-containing immune complexes to the cells and can exaggerate the measured number of Fc receptors. Such cell-bound RF may affect clearance of immune complexes by the reticuloendothelial system in vivo.
通过逆流淘析法纯化了11例类风湿性关节炎患者和10例对照者的单核细胞。类风湿性关节炎单核细胞比对照者的单核细胞具有更多的细胞相关IgG（P<0.001），并且在体外结合更多的125I标记的热聚集IgG（P<0.02）。然后研究了类风湿因子（RF）与单核细胞的相互作用。纯化的125I标记的IgM-RF和IgG-RF直接与正常个体的单核细胞结合。此外，正常单核细胞与RF预孵育增强了随后聚集IgG与细胞的结合。我们得出结论，单核细胞相关的RF可以增强含IgG免疫复合物与细胞的结合，并可以夸大Fc受体的测量数量。这种细胞结合的RF可能会影响体内网状内皮系统对免疫复合物的清除。
