Møller Rasmussen J, Brandslund I, Rasmussen G G, Svehag S E
Scand J Immunol. 1982 Oct;16(4):279-85. doi: 10.1111/j.1365-3083.1982.tb00724.x.
The number of free Fc receptors (FcR) per cell and the association constant (Kass) for the binding of monomeric IgG were determined for monocyte-enriched peripheral blood mononuclear cells, isolated from 16 patients with active classical rheumatoid arthritis (RA) and from 15 normal healthy donors. The assay system was based on binding under equilibrium conditions of 125I-labelled monomeric rabbit IgG to monocytes purified from peripheral blood on a continuous gradient of Percoll. Monocytes from 14 untreated RA patients (6 seropositive, 8 seronegative) expressed on the average 4.8 +/- 1.3 x 10(4) FcR/cell. This number was significantly higher (P less than 0.001) than that found in the control group (34. +/- 0.7 x 10(4) FcR/cell). There was also a significant difference between the mean Kass of the RA group and the control group--2.1 +/- 0.7 x 10(8) l/mol and 2.6 +/- 1.0 x 10(8) l/mol, respectively (0.05 greater than P greater than 0.01). Two seropositive RA patients receiving systemic treatment with penicillamine expressed the same number of FcR/cell as the mean of the control group (3.6 x 10(4)). Levels of circulating immune complexes (CIC) and the complement-factor C3 split product C3d were also measured. No correlation was found between the number of FcR/cell and the concentration of C3d, but there was a weak correlation between the number of FcR/cell and the level of CIC.
从16例活动性典型类风湿性关节炎（RA）患者及15名正常健康供体中分离出富含单核细胞的外周血单核细胞，测定了每个细胞上的游离Fc受体（FcR）数量以及单体IgG结合的缔合常数（Kass）。检测系统基于在平衡条件下，将125I标记的单体兔IgG与通过Percoll连续梯度从外周血中纯化的单核细胞进行结合。14例未经治疗的RA患者（6例血清阳性，8例血清阴性）的单核细胞平均每个细胞表达4.8±1.3×10⁴个FcR。这个数量显著高于对照组（3.4±0.7×10⁴个FcR/细胞）（P＜0.001）。RA组与对照组的平均Kass也存在显著差异，分别为2.1±0.7×10⁸ l/mol和2.6±1.0×10⁸ l/mol（0.05＞P＞0.01）。两名接受青霉胺全身治疗的血清阳性RA患者每个细胞表达的FcR数量与对照组平均值相同（3.6×10⁴）。还测量了循环免疫复合物（CIC）水平和补体因子C3裂解产物C3d。未发现每个细胞的FcR数量与C3d浓度之间存在相关性，但每个细胞的FcR数量与CIC水平之间存在弱相关性。
