School of Public Health, Hebei Medical University, Shijiazhuang, 050017, China; Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environment and Operational Medicine, Tianjin, 300050, China.
Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environment and Operational Medicine, Tianjin, 300050, China.
Anal Chim Acta. 2022 Aug 22;1222:340178. doi: 10.1016/j.aca.2022.340178. Epub 2022 Jul 19.
Kanamycin (KAN) residues in animal-derived food can cause serious threats to human health. Herein, a colorimetric aptasensor was described using "three-in-one" nanohybrids (hemin@Fe-MIL-88NH/PtNP) as synergistic nanozymes assisted with the exonuclease I (Exo I) signal amplification for the ultrasensitive detection of KAN. In the presence of KAN and Exo I, the KAN aptamer (APT) was specifically bound to KAN, and the remaining APT complementary strand (cDNA) captured hemin@Fe-MIL-88NH/PtNP labeled with the cDNA complementary strand (cDNA). Due to the synergistic effect of hemin, Fe-MIL-88NH and platinum nanoparticles (PtNPs), hemin@Fe-MIL-88NH/PtNP exhibited superior catalytic performance and could efficiently catalyze the 3,3',5,5'-tetramethylbenzidine (TMB)-HO system for signal development. Moreover, Exo I could digest APT and release KAN into a new cycle for signal amplification. Based on multiple signal amplification effects, our proposed aptasensor achieved a wide detection range from 0.01 to 100 ng mL with a low detection limit of 2 pg mL. This assay also successfully detected KAN-added milk and shrimp samples with added recovery ranges of 93.58%-106.08% and relative standard deviations (RSDs) of 2.20%-5.50%. The aptasensor enabled ultrasensitive, specific, and fast detection of KAN, and exhibited promising applications in the efficient detection of food pollutants.
卡那霉素（KAN）残留于动物源性食品中会对人类健康造成严重威胁。本研究构建了一种比色适体传感器，以“三位一体”纳米杂化材料（血红素@Fe-MIL-88NH/PtNP）作为协同纳米酶，并结合外切酶 I（Exo I）信号放大，用于超灵敏检测卡那霉素。在 KAN 和 Exo I 的存在下，卡那霉素适体（APT）特异性结合 KAN，剩余的 APT 互补链（cDNA）捕获与 cDNA 互补链（cDNA）标记的血红素@Fe-MIL-88NH/PtNP。由于血红素、Fe-MIL-88NH 和铂纳米粒子（PtNPs）的协同作用，血红素@Fe-MIL-88NH/PtNP 表现出优异的催化性能，并能有效地催化 3,3',5,5'-四甲基联苯胺（TMB）-HO 体系进行信号开发。此外，Exo I 可以消化 APT 并将 KAN 释放到新的循环中进行信号放大。基于多重信号放大效应，我们提出的适体传感器实现了从 0.01 到 100ng mL 的宽检测范围，检测限低至 2pg mL。该方法还成功地检测了添加 KAN 的牛奶和虾样品，加标回收率在 93.58%-106.08%之间，相对标准偏差（RSD）为 2.20%-5.50%。该适体传感器实现了 KAN 的超灵敏、特异、快速检测，在食品污染物的高效检测中具有广阔的应用前景。
