Center for Reproductive Medicine, Peking University Third Hospital, Haidian District, Beijing, the People's Republic of China; Key Laboratory of Reproductive Genetics (Ministry of Education) and Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, the People's Republic of China.
Department of Obstetrics and Gynaecology, Monash University, Clayton, Victoria, Australia.
Fertil Steril. 2022 Sep;118(3):513-521. doi: 10.1016/j.fertnstert.2022.06.003. Epub 2022 Aug 5.
To investigate the relationship between the embryo frozen time and live birth rate (LBR) in women having a freeze-all cycle.
Retrospective cohort study.
Academic hospital.
PATIENT(S): Women who underwent their first vitrified-warmed cycles from January 2013 to December 2019.
INTERVENTION(S): Embryo storage time.
MAIN OUTCOME MEASURE(S): The primary outcome was the LBR.
RESULT(S): A total of 14,928 women were eligible for the analysis. Women with the frozen time of transferred embryos for 2-5 months were associated with a higher LBR compared with other groups. The results were confirmed by an inverted U curve in the restricted cubic splines before as well as after adjustment for covariables, which suggested that an embryo storage time of 3-4 months was associated with the highest LBR. Subgroup analyses demonstrated that the inverted U curve relationship between embryo storage time and LBR was only observed in women with the high response. Sensitivity analyses in women with at least one good-quality embryo for transfer, in women aged <36 years at embryo transfer, or in women with double cleavage embryo transfer showed that the association remained valid. The association was weakened in women with single blastocyst transfer probably because of the small sample size in these women.
CONCLUSION(S): An inverted U-shaped relationship was found between embryo storage time and treatment success in women with high ovarian response in freeze-all embryo transfer cycles. Prolonged storage time of >6 months was associated with reduced pregnancy rates.
研究冻融胚胎保存时间与全胚胎冷冻周期活产率（LBR）的关系。
回顾性队列研究。
学术医院。
2013 年 1 月至 2019 年 12 月接受首次玻璃化冷冻解冻周期的患者。
胚胎储存时间。
主要结局为 LBR。
共有 14928 名女性符合分析条件。与其他组相比，移植胚胎冷冻时间为 2-5 个月的女性活产率更高。在调整协变量前后，受限立方样条的倒 U 曲线均证实了这一结果，这表明胚胎储存时间为 3-4 个月与最高活产率相关。亚组分析表明，胚胎储存时间与 LBR 之间的倒 U 曲线关系仅在高反应患者中观察到。在至少有一个优质胚胎可供移植的患者、胚胎移植时年龄<36 岁的患者或双卵裂胚胎移植的患者中进行敏感性分析显示，该相关性仍然有效。由于这些女性样本量较小，在仅进行单囊胚移植的女性中，这种关联减弱。
在全胚胎冷冻周期中，高卵巢反应患者的胚胎储存时间与治疗成功呈倒 U 型关系。储存时间过长（>6 个月）与妊娠率降低有关。
