Reproductive Medicine Center, Shunde Hospital of Southern Medical University (The First People's Hospital of Shunde), Foshan, Guangdong, China.
Reproductive Medicine Center, Zhuhai Maternal and Childcare Service Center, Zhuhai, Guangdong, China.
Front Endocrinol (Lausanne). 2023 Mar 14;14:1148411. doi: 10.3389/fendo.2023.1148411. eCollection 2023.
With the refinement of cryopreservation technology, the number of frozen-warmed embryo transfer (FET) cycles and cryopreserved embryos has increased rapidly. However, studies investigating the effect of storage duration on pregnancy outcomes after vitrification are limited and their results are controversial. Furthermore, the available studies did not take patients' demographic nor clinical treatment characteristics into account and the cryo-storage duration was short. So this study aimed to explore the effect of storage duration of vitrified warmed embryos on pregnancy and neonatal outcomes in patients with good prognosis and long storage duration of vitrified embryos.
This study was a bi-centre, retrospective study including 1037 women undergoing their first FET cycles following a fresh cycle from January 2012 until December 2021. Patients were divided into four storage groups in accordance with the storage duration of transferred embryos (612 patients in group I, with storage duration between 1 and 6 months; 202 patients in group II, with storage duration between 7 and 12 months; 141 patients in group III, with storage duration between 13 and 36 months; and 76 patients in group IV, with storage duration between 37 and 84 months). The pregnancy and neonatal outcomes were compared amongst different storage duration groups.
Amongst the different groups, no significant differences were observed in the pregnancy outcomes, including biochemical pregnancy rate, implantation rate, clinical pregnancy rate, ongoing pregnancy rate and live birth rate. In addition, no evidence of differences amongst different storage duration groups was observed in terms of preterm birth, birth length and low birthweight.
The pregnancy and neonatal outcomes of embryos after vitrification were not impaired by storage duration up to 7 years.
随着冷冻保存技术的不断完善，冷冻解冻胚胎移植（FET）周期和冷冻胚胎的数量迅速增加。然而，研究胚胎玻璃化后储存时间对妊娠结局影响的研究有限，结果存在争议。此外，现有研究没有考虑患者的人口统计学和临床治疗特征，且冷冻储存时间较短。因此，本研究旨在探讨预后良好且冷冻胚胎储存时间较长的患者中，玻璃化解冻胚胎储存时间对妊娠和新生儿结局的影响。
本研究是一项双中心回顾性研究，纳入了 2012 年 1 月至 2021 年 12 月期间首次进行新鲜周期 FET 周期的 1037 名妇女。根据转移胚胎的储存时间，患者被分为四个储存组：I 组 612 例，储存时间 1-6 个月；II 组 202 例，储存时间 7-12 个月；III 组 141 例，储存时间 13-36 个月；IV 组 76 例，储存时间 37-84 个月。比较不同储存时间组的妊娠和新生儿结局。
在不同组中，生化妊娠率、种植率、临床妊娠率、持续妊娠率和活产率等妊娠结局均无显著差异。此外，不同储存时间组在早产、出生长度和低出生体重方面也没有差异的证据。
胚胎玻璃化后储存时间长达 7 年，对妊娠和新生儿结局没有影响。
